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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/TransforMax™ EC100™ Chemically Competent E. coli/CC02810/10 x 50 µl

Lucigen/TransforMax™ EC100™ Chemically Competent E. coli/CC02810/10 x 50 µl

价格
￥2600.00
货号：CC02810
浏览量：127
品牌：Lucigen
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商品描述

Generalcloningstrain

  • Formerly from EpicentreClonelargeinsertsandtransformlargeplasmids-uptoatleast145kbplasmidDNA.
  • Achievehighefficiencieswithconvenientchemicallycompetentformat:>5×108cfu/µgpUC19DNA.
  • Idealforcloning,subcloningandplasmidpreparationduetoendAandrecAmutations.

Applications

  • RoutinecloningofDNAupto200kb.

ThehighlyversatileTransforMax™EC100™E.colicompetentcellsareidealformostcloningapplications.ThecellsprovideveryhightransformationefficiencywhentestedagainstawiderangeofsupercoiledDNAsaswellasDNAdirectlyfromaligationreaction(Table1).

Benefits

  • Hightransformationefficiencywithclonesofallsizes,includingBACclones(Table 1).
  • lacZΔM15forblue/whitescreeningofrecombinants.
  • Restrictionminus[mcrA,Δ(mrr-hsdRMS-mcrBC)]enablesefficientcloningofmethylatedDNA.
  • Endonucleaseminus(endA1)toensurehighyieldsofDNA.
  • Recombinationminus(recA1)forgreaterstABIlityoflargeclonedinserts.
DNATransforMax™EC100™ChemicallyCompetentE. coliTransforMax™EC100™ElectrocompetentE. coli
pUC191.4×1081.4×1010
8.1-kbClone1.3×107Nottested
13.1-kbClone4.3×1061.3×109
23.1-kbClone9.2×1053.0×108
145-kbBACCloneNottested7×107
13.1-kbclonedirectlyfromaligationreaction2.2×1052.1×107

Table1.ComparisonofthetransformationefficienciesofTransforMax™EC100™E. coliwithavarietyofDNAs.Transformationswereperformedusing50µlofcompetentcellsandeithersupercoiledDNAsoftheindicatedsizesora1-µlaliquotfromastandard10-µlligationreaction.Resultsshownareincfu/µgofDNAandaretheaveragetransformationefficienciesobtainedfromseveraltrials.

Genotype

F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupG

TransforMaxEC100ElectrocompetentE.coli

  • Transformationefficiencyof>1×1010cfu/µgofpUC19.

TransforMaxEC100ChemicallyCompetentE.coli

  • Transformationefficiencyof>5×108cfu/µgofpUC19.

ORDERINFORMATION

IncludespUC19controlDNA. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限，在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应，您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量，可靠性和稳定的性能。