品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

Lucigen/DNATerminator® End Repair Kit/40035-2/50 rxns

价格
¥6540.00
货号:40035-2
浏览量:127
品牌:Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • Repairshearedorrestriction-digestedDNAends
  • Increasecloningefficiencyasmuchasa500%
  • Optimizedsystemforconsistent&reliableresults

RepairDNAends

Double-strandedDNAfragmentsforlibraryconstructionorlarge-scalesequencingareoftengeneratedbymechanicallyshearinglarger(genomic)DNA,aprocessthatprimarilyleavesunevenends.LargeDNAmayalsobecutwithrestrictionenzymestogeneratesmallerfragmentsforsubcloning.ShearedorrestrictedDNAfragmentsmustbe“end-repaired”beforeligationintoblunt-endcloningvectors(e.g.,Lucigen’sCloneSmart®,CopyRight®,orBigEasy®systems).

TheDNATerminatorEndRepairKitcreatesblunt,5'phosphorylatedendsonanytypeofshearedorrestriction-digestedDNAfragment,ensuringthehighestpossIBLecloningefficiency(Figure1).

DNA Terminator Kit

Figure1.EffectofDNAshearingandend-repairmethodsonlibraryconstructionefficiency.Shotgunlibrarieswereconstructedusing250ngoflamBDaDNAshearedbysonication(Son)orbytheGeneMachines®HydroShear™device(HS).ShearedDNAwasrepairedwithT4DNApolymeraseandKlenowfragment(TK)orDNATerminatorEndRepairKit(DT)andclonedusingLucigen’sCloneSmart®system.Valuesarecolonyformingunits(cfu)perlibrary.

ORDERINFORMATION

TheDNATerminatorEndRepairKitcontains:DNATerminatorEndRepairEnzymeMix,EndRepairBuffer,andacompleteprotocol. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。