- Ligationreactionistransformation-readyinminutes
- NoDNAcleanupsteprequired
- HighlyreliableandreproducIBLeresults
Quickencloning
TheCloneDirectRapidLigationKitisasimple,rapid,highlyefficientalternativetostandardligationandtransformationprotocols.Thiskiteliminatestherequirementforapost-ligationcleanupstepbeforeelectroporation.A15-minuteheatdenaturationat70°Cisallthatisneededpriortocelltransformation.
Figure1.Ligationefficienciesmeasuredbytransformation.Ligationreactionswereheatdenaturedat70°CandusedwithoutadditionalcleanuptotransformelectrocompetentE.cloni®10Gcells.Transformants(inmillions)areshown. |