MaximizedpackagingefficiencyoflamBDa,cosmid,orfosmidDNAfortheconstructionoflargeinsertgenomiclibraries.
- ConstructgenomiclibrariesfrommethylatedorunmethylatedDNAwithequalefficiencyduetothelackofallknownrestrictionactivitiesintheextracts.
- Packagingefficienciesofupto3×109pfu/µgDNA.
- Simplifiedprotocoldoesnotrequirepremixingofdifferentextractcomponentsbeforeuse.
MaxPlax™LambdaPackagingExtractsoffermaximuminvitropackagingefficienciesofcossite-containingmethylatedorunmethylatedDNA.TrADItionallambdapackagingextractsarederivedfromtwocomplementarylysogenicE.colistrains,BHB2690andBHB2688,asdescribedbyHohn.1MaxPlaxExtractsusearestriction-freeE.coliK-12strain,NM759,inplaceofstrainBHB2690.2ThesonicatedextractsofNM759arecombinedwithextractsofBHB2688toproduceMaxPlaxExtracts.
EachlotofMaxPlaxExtractsistestedandguaranteedtomaintainapackagingefficiencyofgreaterthan1×109pfu/µgofControlLigatedLambdaDNAfor1yearfromthedateofpurchaseifstoredproperly.ACertificateofAnalysisstatingactualpackagingefficiency(pfu/µgDNA)isprovidedwitheachlotofMaxPlaxExtracts.
Table1.PackagingofmethylatedandunmethylatedDNAusingMaxPlax™LambdaPackagingExtracts. | |
CosmidInsert | Pfu/µgDNA |
UnmethylatedDNA | 3.5x107 |
MethylatedDNA | 3.9x107 |
References
- Hohn,E.G.(1979)MethodsEnzymol.68,299.
- Gunther,E.G.etal.(1993)NucleicAcidsRes.21,3903.
- Fiandt,M.(2000)EpicentreForum7(3),14.