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Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts

价格
¥3860.00
货号:MP5105
浏览量:127
品牌:Lucigen
服务
全国联保
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商品描述

MaximizedpackagingefficiencyoflamBDa,cosmid,orfosmidDNAfortheconstructionoflargeinsertgenomiclibraries.

  • Formerly from EpicentreConstructgenomiclibrariesfrommethylatedorunmethylatedDNAwithequalefficiencyduetothelackofallknownrestrictionactivitiesintheextracts.
  • Packagingefficienciesofupto3×109pfu/µgDNA.
  • Simplifiedprotocoldoesnotrequirepremixingofdifferentextractcomponentsbeforeuse.

MaxPlax™LambdaPackagingExtractsoffermaximuminvitropackagingefficienciesofcossite-containingmethylatedorunmethylatedDNA.TrADItionallambdapackagingextractsarederivedfromtwocomplementarylysogenicE.colistrains,BHB2690andBHB2688,asdescribedbyHohn.1MaxPlaxExtractsusearestriction-freeE.coliK-12strain,NM759,inplaceofstrainBHB2690.2ThesonicatedextractsofNM759arecombinedwithextractsofBHB2688toproduceMaxPlaxExtracts.

EachlotofMaxPlaxExtractsistestedandguaranteedtomaintainapackagingefficiencyofgreaterthan1×109pfu/µgofControlLigatedLambdaDNAfor1yearfromthedateofpurchaseifstoredproperly.ACertificateofAnalysisstatingactualpackagingefficiency(pfu/µgDNA)isprovidedwitheachlotofMaxPlaxExtracts.

Table1.PackagingofmethylatedandunmethylatedDNAusingMaxPlax™LambdaPackagingExtracts.
CosmidInsertPfu/µgDNA
UnmethylatedDNA3.5x107
MethylatedDNA3.9x107

References

  1. Hohn,E.G.(1979)MethodsEnzymol.68,299.
  2. Gunther,E.G.etal.(1993)NucleicAcidsRes.21,3903.
  3. Fiandt,M.(2000)EpicentreForum7(3),14.

ORDERINFORMATION

Contents:PredispensedMaxPlax™Extracts,ControlLambdaDNA,ControlHostCells(E.coli). Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。