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Lucigen/TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip/TAN091096/96 Reactions

价格
¥52140.00
货号:TAN091096
浏览量:127
品牌:Lucigen
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商品描述

Optimizedforhighsignalintensity

  • Formerly from EpicentreFast.Performthe6-hourreactionandbeginBeadChiphybridizationthesameday.
  • HighSensitivity.SensitiveandreproducIBLegenedetection.
  • HighYield.Makemicrogramamountsofbiotin-aRNA(biotin-cRNA)fromaslittleas25ngoftotalRNA.
  • HighFidelity.TargetAmp-Nanolabelingprocedurepreservestherelativeabundanceoftheoriginalsample.

TheTargetAmp™-NanoLabelingKitproducesbiotin-aRNA(alsocalledbiotin-cRNA)thathasbeenoptimizedforhighsignalintensityonIlluminaExpressionBeadChiparrays,enablingsensitivegenedetectionandreproducibledifferentialexpressionresults.

Benefits

  • Fast.Performthe6-hourreactionandbeginBeadChiphybridizationthesameday.
  • HighSensitivity.Sensitiveandreproduciblegenedetection.
  • HighYield.Makemicrogramamountsofbiotin-aRNA(biotin-cRNA)fromaslittleas25ngoftotalRNA.
  • HighFidelity.TargetAmp-Nanolabelingprocedurepreservestherelativeabundanceoftheoriginalsample.

Table1.TargetAmp-NanoKitaGuide

InputRNAReaction
Time
Additional
TargetAmp-NanoKit25ng-500ngtotalRNA6hoursRequiresuseofSuperScriptIIIReverseTranscriptase
(providedbytheuser)

Table2.YieldofBiotin-aRNAfromTargetAmp-NanoKit

InputRNA(HeLatotalRNA)50pg100pg500pg25ng100ng500ng
TargetAmp-NanoKitreaction3μg10μg75μg

ORDERINFORMATION

ThesekitswereoptimizedusingSuperScript™IIIreversetranscriptase(Invitrogen),whichisnotincludedinthekits.Contents:TargetAmp™T7-Oligo(dT)Primer,TargetAmp™Nano1st-StrandCDNAPremix,TargetAmp™Nano2nd-StrandcDNAPremix,TargetAmp™Nano2nd-StrandcDNADNAPolymerase,TargetAmp™NanoT7RNAPolymerase,TargetAmp™NanoT7TranscriptionBuffer,TargetAmp™NanoNTPPreMix,TargetAmp™NanoUTP/Biotin-UTP,RNase-FreeDNaseI,DTT,HeLaTotalRNAControl,RNase-FreeWater. Lucigen基因组编辑和工程面临许多挑战。为您的编辑实验获取可靠的试剂不应该是其中之一。无论您是在突破CRISPR技术的界限,在体内产生基因敲除或敲入还是在进行序列消化或鉴定的体外反应,您都需要可靠的工具和酶。依靠CRISPRcraft™获得质量,可靠性和稳定的性能。