Optimizedforhighsignalintensity
- Fast.Performthe6-hourreactionandbeginBeadChiphybridizationthesameday.
- HighSensitivity.SensitiveandreproducIBLegenedetection.
- HighYield.Makemicrogramamountsofbiotin-aRNA(biotin-cRNA)fromaslittleas25ngoftotalRNA.
- HighFidelity.TargetAmp-Nanolabelingprocedurepreservestherelativeabundanceoftheoriginalsample.
TheTargetAmp™-NanoLabelingKitproducesbiotin-aRNA(alsocalledbiotin-cRNA)thathasbeenoptimizedforhighsignalintensityonIlluminaExpressionBeadChiparrays,enablingsensitivegenedetectionandreproducibledifferentialexpressionresults.
Benefits
- Fast.Performthe6-hourreactionandbeginBeadChiphybridizationthesameday.
- HighSensitivity.Sensitiveandreproduciblegenedetection.
- HighYield.Makemicrogramamountsofbiotin-aRNA(biotin-cRNA)fromaslittleas25ngoftotalRNA.
- HighFidelity.TargetAmp-Nanolabelingprocedurepreservestherelativeabundanceoftheoriginalsample.
Table1.TargetAmp-NanoKitaGuide
InputRNA | Reaction Time | Additional | |
TargetAmp-NanoKit | 25ng-500ngtotalRNA | 6hours | RequiresuseofSuperScriptIIIReverseTranscriptase (providedbytheuser) |
Table2.YieldofBiotin-aRNAfromTargetAmp-NanoKit
InputRNA(HeLatotalRNA) | 50pg | 100pg | 500pg | 25ng | 100ng | 500ng |
TargetAmp-NanoKitreaction | 3μg | 10μg | 75μg |