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蚂蚁淘在线 / 品牌中心 / Hycult Biotech / Hycult Biotech/C4d, Human, mAb 12D11, immuno assay antibody/HM2229-IA/0.5 mg
商品描述
Description:C4d,Human,mAb12D11,immunoassayantibody
Themonoclonalantibody12D11(alsoknownascloneM4d3)recognizesanepitopeonthealpha2chainofhumanC4d.ComplementfactorC4(MW41kDa),formerlyknownasGgprotein,consistsofanalpha-,beta-andgamma-chain.Theclassicalpathwayofcomplementandthemannosebindinglectin(MBL)activationpathwayconvergeatC4.ActivatedC1,MASP-1andMASP-2cleaveC4resultingintheformationofC4aandC4b.ThelattercanbecleavedbyfactorIresultinginC4candC4d,inwhichstepallfunctionalsitesarelost.TheC4dactivationfragmentofC4isanexcellentMarkerforclassicalcomplementpathwayandMBLpathwayactivation.ThethioesterformedbetweenthesidechainsofCys1010andGln1013withintheC4dregionoftheα-chainmediatescovalentattachmenttothetargetsurfacebearingactivatedformsofC1sorMASP.
FurThermore,C4dishighlyhomologoustoC3dwithover35%sharedaminoacidsequence.Inanumberofdiseasessuchasrheumatoidarthritis(RA),hereditaryangioedema(HAE),systemiclupuserythematosus(SLE)andchronicurticariawithhypercomplementemialevelsofC4daresignificantlyelevatedinserumorplasma.C4dlevelsmayalsobeelevatedinplasmafrompatientswithavarietyofhumoralautoimmunediseasesinwhichcomplementactivationisknowntooccur.DepositionofC4dinperitubularcapillarieshasbeenshowntobeasensitivemarkerforantibody-mediated(humoral)rejectioninrenaltransplantbiopsies.Themonoclonalantibody12D11recognizesC4,C4bandC4d.Antibody12D11crossreactswithpig,bovine,baboonandrhesusmonkey.
FurThermore,C4dishighlyhomologoustoC3dwithover35%sharedaminoacidsequence.Inanumberofdiseasessuchasrheumatoidarthritis(RA),hereditaryangioedema(HAE),systemiclupuserythematosus(SLE)andchronicurticariawithhypercomplementemialevelsofC4daresignificantlyelevatedinserumorplasma.C4dlevelsmayalsobeelevatedinplasmafrompatientswithavarietyofhumoralautoimmunediseasesinwhichcomplementactivationisknowntooccur.DepositionofC4dinperitubularcapillarieshasbeenshowntobeasensitivemarkerforantibody-mediated(humoral)rejectioninrenaltransplantbiopsies.Themonoclonalantibody12D11recognizesC4,C4bandC4d.Antibody12D11crossreactswithpig,bovine,baboonandrhesusmonkey.
Specifications:
| Catalognumber | HM2229-IA |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBS(exactconcentrationisindicatedonthelabel). |
| Application | Flowcytometry,Frozensections,Immunoassays,Westernblot |
| ApplicationNotes | F:PermeABIlizationwasdoneincoldacetonewith0.5%hydrogenperoxidasefor10min,afterdryingandwashing,antibodieswereincubatedfor30minutes. FC:Pneumococciwereincubatedwith25μlofa1:10diluted12D11,incubatedfor30minutesandwashed(2x),followedby30minutesincubationoniceofa1:100dilutedgoat-anti-mouseIgGPEoniceinthedarkandwashed(2x)(Ref.5). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Isotype | MouseIgG1 |
| Species | Human |
| Crossreactivity | Baboon-Yes,Bovine-Yes,Rhesusmonkey-Yes,Swine-Yes |
| References | 1.Zwirner,Jetal;Classicalpathwayofcomplementactivationinnormalanddiseasedhumanglomeruli.KidneyInt1989,36:1069. 2.Zwirner,Jetal;Classicalpathwayofcomplementactivationinmammaliankidneys.Immunology1993,80:162. 3.Feucht,Hetal;CapillarydepositionofC4dcomplementfragmentandearlyrenalgraftloss.KidneyInt1993,43:1333. 4.Rensen,Setal;ActivationoftheComplementSysteminHumanNonalcoholicFattyLiverDisease.Hepatology2009,50:1809 5.Meijvis,Setal;Strategiestoimproveclinicalmanagementofcommunity-acquiredpneumonia.Thesis2012. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Infectiousdiseases,Nephrology |
