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Hycult Biotech/TNF-alpha, Human, mAb 52B83/HM2010/100 µg

价格
¥6960.00
货号:HM2010
浏览量:127
品牌:Hycult Biotech
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商品描述

Description:TNF-alpha,Human,mAb52B83

Themonoclonalantibody52B83reactswithtumornecrosisfactoralpha(TNF-alpha).TNF-alphaisahomotrimeric17kDaprotein,thatinteractswitheitheroneofthetwotypesofTNF-receptors,termedIandII,leADIngtoreceptorcross-linkingandsignaltransduction.Thereceptorsdifferstronglyintheirintra-cellularsignalingpathways.
TNF-alphawasoriginallydescribedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,-TNF-alphaisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses.Itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesleadingtoexpressionofawidevarietyof-cytokines.Thepro-inflammatorypropertiesof-TNF-alphaplayacentralroleinseveralauto-immunediseasessuchasrheumatoidarthritisandinhibitionbyneutralizingmoleculeshavebeenshowntobebeneficialinpatients.

Specifications:

CatalognumberHM2010
ProducttypeMonoclonalantibodies
Quantity100µg
Formulation1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide.
ApplicationFlowcytometry,Frozensections,Immunoassays,Westernblot
ApplicationNotesFC:Antibody52B83wasusedtostainsolubleTNFboundtoTNFreceptors.CellswerefixedinPBAcontaining0.2%formaldehyde(Ref.3)
W:Areducedsampletreatmentand15%SDS-Pagewasused.Thebandsizeobservedis17kDa(Ref.4).
IHC-F:6-μmtissuesectionswerefixedinacetonefor10minutes(Ref.4).
UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.
IsotypeMouseIgG1
SpeciesHuman
CrossreactivityGuineapig-Yes,Mouse-Yes,Rhesusmonkey-Yes
AliasTumorNecrosisFactor-alpha,TNF,TNF-SF2,cachectin
References1.Bradding,Petal;Interleukin-4,-5,and-6andTumorNecrosisFactor-alphainnormalandasthmaticairways:Evidenceforthehumanmastcellasasourceofthesecytokines.AmJRespirCellMolBiol1994,10:471
2.Bradding,Petal:TNFalphaislocalizedtonasalmucosalmastcellsandisreleasedinacuteallergicrhinitis.ClinExpAllergy1995,25:406
3.Gerspach,Jetal;Detectionofmembrane-boundTumorNecrosisFactor(TNF):ananalysisofTNF-specificreagents.MicroscResTech2000,50:243
4.Laanvander,Netal;Tumornecrosisfactoralpha(TNFalpha)inhumanskin:acomparisonofdifferentantibodiesforimmunohistochemistry.ArchDermatolRes2001,293:226
5.Lee,Eetal;Clinicalandimmunohistochemicalcharacteristicsofmucoceles.AnnDermatol2009,21:345
6.Brunner,Petal;InfliximabinducesdownregulationoftheIL-12/IL-23axisin6-sulfo-LacNac(slan)1dendriticcellsandmacrophages.JAllergyClinImmunol2013,132:1184
StorageandstABIlityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.
PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct.
DiseaseAutoimmunity,Gastroenterology
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