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商品描述
Description:TNF-alpha,Human,mAb52B83
Themonoclonalantibody52B83reactswithtumornecrosisfactoralpha(TNF-alpha).TNF-alphaisahomotrimeric17kDaprotein,thatinteractswitheitheroneofthetwotypesofTNF-receptors,termedIandII,leADIngtoreceptorcross-linkingandsignaltransduction.Thereceptorsdifferstronglyintheirintra-cellularsignalingpathways.
TNF-alphawasoriginallydescribedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,-TNF-alphaisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses.Itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesleadingtoexpressionofawidevarietyof-cytokines.Thepro-inflammatorypropertiesof-TNF-alphaplayacentralroleinseveralauto-immunediseasessuchasrheumatoidarthritisandinhibitionbyneutralizingmoleculeshavebeenshowntobebeneficialinpatients.
TNF-alphawasoriginallydescribedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,-TNF-alphaisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses.Itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesleadingtoexpressionofawidevarietyof-cytokines.Thepro-inflammatorypropertiesof-TNF-alphaplayacentralroleinseveralauto-immunediseasessuchasrheumatoidarthritisandinhibitionbyneutralizingmoleculeshavebeenshowntobebeneficialinpatients.
Specifications:
Catalognumber | HM2010 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
Application | Flowcytometry,Frozensections,Immunoassays,Westernblot |
ApplicationNotes | FC:Antibody52B83wasusedtostainsolubleTNFboundtoTNFreceptors.CellswerefixedinPBAcontaining0.2%formaldehyde(Ref.3) W:Areducedsampletreatmentand15%SDS-Pagewasused.Thebandsizeobservedis17kDa(Ref.4). IHC-F:6-μmtissuesectionswerefixedinacetonefor10minutes(Ref.4). |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Isotype | MouseIgG1 |
Species | Human |
Crossreactivity | Guineapig-Yes,Mouse-Yes,Rhesusmonkey-Yes |
Alias | TumorNecrosisFactor-alpha,TNF,TNF-SF2,cachectin |
References | 1.Bradding,Petal;Interleukin-4,-5,and-6andTumorNecrosisFactor-alphainnormalandasthmaticairways:Evidenceforthehumanmastcellasasourceofthesecytokines.AmJRespirCellMolBiol1994,10:471 2.Bradding,Petal:TNFalphaislocalizedtonasalmucosalmastcellsandisreleasedinacuteallergicrhinitis.ClinExpAllergy1995,25:406 3.Gerspach,Jetal;Detectionofmembrane-boundTumorNecrosisFactor(TNF):ananalysisofTNF-specificreagents.MicroscResTech2000,50:243 4.Laanvander,Netal;Tumornecrosisfactoralpha(TNFalpha)inhumanskin:acomparisonofdifferentantibodiesforimmunohistochemistry.ArchDermatolRes2001,293:226 5.Lee,Eetal;Clinicalandimmunohistochemicalcharacteristicsofmucoceles.AnnDermatol2009,21:345 6.Brunner,Petal;InfliximabinducesdownregulationoftheIL-12/IL-23axisin6-sulfo-LacNac(slan)1dendriticcellsandmacrophages.JAllergyClinImmunol2013,132:1184 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Autoimmunity,Gastroenterology |