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商品描述
Description:C4d,Rat,pAb
ThepolyclonalantibodyrecognizesratC4d.ComplementfactorC4(MW41kDa),formerlyknownasGgprotein,consistsofanalpha-,beta-andgamma-chain.Theclassicalpathwayofcomplementandthemannosebindinglectin(MBL)activationpathwayconvergeatC4.Activated,MASP-1andMASP-2cleaveC4resultingintheformationofC4aandC4b.ThelattercanbecleavedbyfactorIresultinginC4candC4d,inwhichstepallfunctionalsitesarelost.TheC4dactivationfragmentofC4isanexcellentMarkerforclassicalcomplementpathwayandMBLpathwayactivation.ThethioesterformedbetweenthesidechainsofCys1010andGln1013withintheC4dregionoftheα-chainmediatescovalentattachmenttothetargetsurfacebearingactivatedformsofC1sorMASP.FurThermore,C4dishighlyhomologoustoC3dwithover35%sharedaminoacidsequence.Inanumberofdiseasessuchasrheumatoidarthritis(RA),hereditaryangioedema(HAE),systemiclupuserythematosus(SLE)andchronicurticariawithhypercomplementemialevelsofC4daresignificantlyelevatedinserumorplasma.C4dlevelsmayalsobeelevatedinplasmafrompatientswithavarietyofhumoralautoimmunediseasesinwhichcomplementactivationisknowntooccur.DepositionofC4dinperitubularcapillarieshasbeenshowntobeasensitivemarkerforantibody-mediated(humoral)rejectioninrenaltransplantbiopsies.
Specifications:
| Catalognumber | HP8034 |
|---|---|
| Producttype | Polyclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections,Paraffinsections,Westernblot |
| Use | Forimmunohistochemistry,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Asynthesized14-merpeptide(STPAPRNPSEPVPQ)correspondingtoaminoacids1223-1236ofratC4,conjugatedtokeyholelimpethemocyaninattheC-terminalpart(forthisacysteinewasadded) |
| Isotype | RabbitIg |
| Species | Rat |
| References | 1.Minami,Ketal;C4ddepositionandclearanceincardiactransplantscorrelateswithalloantibodylevelsandrejectioninrats.AmJTransplant2006,6:923 2.Qian,Zetal;Antibodyandcomplementmediatedinjuryintransplantsfollowingsensitizationbyallogeneicbloodtransfusion.Transplant2006,82:857 3.Asano,H,etal;Treatmentwithriboflavinandultravioletlightpreventsalloimmunizationtoplatelettransfusionsandcardiactransplants.Transplant2007,84:1174 4.Murata,K,etal;C4ddepositionandcellularinfiltratesasmarkersofacuterejectioninratmodelsoforthotopiclungtransplantation.Transplant2008,86:123 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Infectiousdiseases,Nephrology |
