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商品描述
Description:Chlorotyrosine,pAb
Thepolyclonalantibodyrecognizes3-chlorotyrosinespecificproteinadductsthatareformedbyhypochlorousacid.PolymorphonuclearleukocytesorneutrophilsareoneoftheprimarydefensemechanismsagainstinvADIngmicroorganismsandalsoinvolvedintheremovalofnecroticcells.However,incertainconditions,recruitmentofneutrophilsmayaggravateexistinginjury.Forexample,neutrophilsareinvolvedinthepathophysiologyofhepaticischemia-reperfusioninjury,endotoxin-andsepsis-inducedliverfailure,alcoholichepatitis,andcertaindrugtoxicities.Thefactthatneutrophilsaccumulateintheliverdoesnotnecessarilymeanthattheycauseinjury.ICAM-1andCD18integrinsareinvolvedintheadherenceofneutrophilstotargetcells,e.g.,hepatocytes.Thisresultsinalong-lastingadherence-dependentoxidantstress,whichisamajorfactorinneutrophil-mediatedlivercellkilling.Stimulatedneutrophilsreleaseoxidants,proteases,andotherpotentiallyinjuriousconstituents.TheygeneratesuperoxideradicalsandhydrogenperoxideandreleaseMPO,whichcatalyzesoxidationofchloridebyhydrogenperoxidetogivehypochlorousacid.Thishypochlorousacidcanreactwithproteinstoform3-chlorotyrosineproteinadducts.AlimitationforinvestigatingthepathophysiologicalroleofneutrophilsinvivoisthelackofareliablebioMarkerforneutrophilcytotoxicity.Immunohistochemicalstainingofliversampleswithanti3-chlorotyrosineantibodycorrelateswellwithneutrophil-inducedliverinjury.Thepolyclonalantibodycanbeusedtoassesschlorotyrosineproteinadductformationandisausefulmarkerofneutrophil-inducedlivercellinjuryinvivo.
Specifications:
Catalognumber | HP5002 |
---|---|
Producttype | Polyclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.02%sodiumazideand0.1%bovineserumalbumin. |
Application | Frozensections,Immunoassays,Paraffinsections |
Use | Forimmunoassaysandimmunohistology,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Isotype | RabbitIg |
Species | N/A |
References | 1.Gujral,Jetal;Chlorotyrosineproteinadductsarereliablebiomarkersofneutrophil-inducedcytotoxicityinvivo.ComparativeHepatology2004,3:S48 2.Gujral,Jetal;FunctionalimportanceofICAM-1inthemechanismofneutrophil-inducedliverinjuryinbileduct-ligatedmice.AmJPhysiolGastrointestLiverPhysiol2004,286:G499 3.Gujral,Jetal;NADPHoxidase-derivedoxidantstressiscrititcalforneutrophilcytotoxicityduringendotoxemia.AmJPhysiolGastrointestLiverPhysiol2004,287:G243 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Cardiologyandmetabolism,Pulmonology |