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苏州工业园区生物纳米园A4#216
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商品描述
Description:SP-B,Pig,pAb
ThepolyclonalantibodyrecognizesthehumansurfactantproteinB(SP-B).Therearefoursurfactant-specificproteins,designatedsurfactantproteinA(SP-A),SP-B,SP-CandSP-Drespectively.SP-AandSP-Darehydrophilicsurfactantproteinsandaremembersofthecollectinfamily.SP-BandSP-Carehydrophobicsurfactantproteinsandmaybethemostappropriateindicatorsfortheevolutionaryoriginofsurfactant.SP-BissynthesizedbythealveolartypeIIepithelialcellsasa40-42kDprecursorthatissubsequentlyproteolyticallyprocessedto7.8-8kD.SP-BenhancesthespreADIngandstABIlityofsurfactantphospholipidsinthealveolus.SP-Bisessentialforair-breathinginmammalsandisthereforelargelyconserved.SP-Bcaninteractwithbothphospholipidheadgroupsandfattychainsandisparticularlyactiveinenhancingsurfaceactivebehaviourinendogenousandexogenouslungsurfactants.EvenlowSP-Bcontentshadmeasurableeffectsinincreasingtheadsorption,dynamicsurfacetensionlowering,and/orfilmrespreadingofDPPC,mixedsyntheticlipids,andcolumn-purifiedlungsurfactantphospholipids.DeficiencyofSP-Bandothersurfactantcomponentsisassociatedwithrespiratorydistresssyndrome(RDS)inprematureinfantsandadultswithrespiratorydistresssyndrome(ARDS).ThepolyclonalantibodyisraisedagainstpigSP-Bfrompiglungswhichhasbeenreconstitutedinmicellesoflysophosphatidylcholine.TheantibodyrescognizesundernonreducingconditionsthemostprominentformsofSP-Bof8kDand22kD,correspondingtoSP-Bdimers.ThepolyclonalantibodyiscrossreactivewithhumanSP-B.
Specifications:
Catalognumber | HP7002 |
---|---|
Producttype | Polyclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
Application | Immunoassays,Westernblot |
Use | ForWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Isotype | RabbitIg |
Species | Swine |
Crossreactivity | Human-Yes |
References | 1.Oviedo,Jetal;QuantitationofpulmonarysurfactantproteinSP-Bintheabsenceorpresenceofphospholipidsbyenzyme-linkedimmunosorbentassay.AnalBiochem2001,293:78 2.Perez-Gil,Jetal;Lipid-proteininteractionsofhydrophobicproteinsSP-BandSP-Cinlungsurfactantassemblyanddynamics.PediatrPatholMolMed2001,20:445 |
Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Pulmonology |