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商品描述
Description:TNF-RI,Mouse,pAb
ThepolyclonalantibodyrecognizestheextracellularpartofthemouseTumorNecrosisFactorReceptortype1(TNF-RI)ofthemembrane-boundaswellasthesolublereceptor.TNF-RI(~55-60kDa)ispresentonmostcelltypesandisconsideredtoplayaprominentroleincellstimulationbyTNF-alpha.TNF-alphaactivatesinflammatoryresponses,inducesapoptosis,regulatescellularproliferation,andmayevenpromotecancerprogression.TheeffectsofTNF-alphaaremediatedbyTNF-RIandTNF-RII,whichhavebothdistinctandoverlappingdownstreamsignalingcascades.InductionofcytotoxicityandotherfunctionsaremediatedlargelyviaTNF-RI.TNF-RIisequallywellactivatedbyboththe17kDasolubleand26kDamembrane-boundform,whereasTNF-RIIisefficientlyactivatedonlybythemembraneboundformofTNF-alpha.TNF-RIsignalingisinitiatedwhentrimericTNF-alphabindsTNF-RIreceptors.SubsequentTNF-RItrimerizationpromotestherecruitmentofaproximalsignalingcomplexcomposedofTNFReceptorAssociatedproteinwithaDeathDomain(TRADD),ReceptorInteractingProtein(RIP),cellularInhibitorofApoptosisProtein1(cIAP1),TNFReceptorAssociatedFactor2(TRAF2),andlikelyTRAF5.StudieswithTNF-RI-deficientmiceindicatethatTNF-RImediatesmostoftheproliferation,pro-inflammatory,andapoptosis-activatingpathways.
Specifications:
Catalognumber | HP8002 |
---|---|
Producttype | Polyclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin |
Application | Flowcytometry,Functionalstudies,Immunoassays,Immunoprecipitation,Westernblot |
Use | ForflowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsetting.AntibodyreactivitywithcellboundorsolubleTNF-RIisnotinhibitedbyhighconcentrationsofmouseTNFα. |
Isotype | RabbitIg |
Species | Mouse |
Alias | Tumornecrosisfactorreceptorsuperfamilymember1A,TNF-RI,p55/p60 |
References | 1.Lucas,Retal;BothTNFreceptorsarerequiredfordirectTNF-mediatedcytotoxicityinmicrovascularendothelialcells.EurJImmunol1998,28:3577 2.Bemelmans,Metal;LPS-InducedsTNF-receptorreleaseinvivoinamurinemodel.JImmunol1993,151:5554 3.Gerspach,Jetal;Detectionofmembrane-boundtumornecrosisfactor(TNF):ananalysisofTNF-specificreagents.MicrResTech2000,50:243 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Autoimmunity,Gastroenterology |