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商品描述
Description:CD166,Human,mAbAZN-L50
ThemonoclonalantibodyAZN-L50recognizesactivatedleukocytecelladhesionmolecule(ALCAM),amemberoftheimmunoglobulinsuperfamilyandhasacharacteristicVVC2C2C2domainstructure.ALCAMisatypeItransmembraneproteinof100kDawithfiveextracellularimmunoglobulin-likedomainsandashortcytoplasmictail.ALCAMmediateshomotypicALCAM-ALCAMinteractionsandheterotypicinteractionswiththeT-cellantigenCD6.Homotypicinteractionsareinvolvedinthedevelopmentandmaintenanceoftissuearchitectureandtumorprogression.HeterotypicinteractionsinitiateandstABIlizeT-cell-dendriticinteractionsaffectingT-cellactivation.
ALCAMissecretedasaNH2-terminalfragmentof30kD.SolubleALCAM(sALCAM)inducesdivergentBIOLOGicalsignalsandresponses.
ALCAMshowsacharacteristic,temporalandspatialdistributionindevelopmentofawidevarietyoftissuesandcellsinhealthanddisease.ALCAMisexpressedonawidevarietyofcells,withintheleukocytepopulationitsexpressionisparticularlyhighondentriticcells.ALCAMisinvolvedinvariousphysiologicalprocessesincludinghematopoieses,thymusdevelopment,theimmuneresponse,neuriteextension,neuralcellmigration,embryogenesis,neurogenesis,angiogenesis,osteogenesisandisaMarkerforpluripotentmesenchymalstemcells.
ThemonoclonalantibodyAZN-L50completelyblockshomophilicALCAM-ALCAMinteraction.
ALCAMissecretedasaNH2-terminalfragmentof30kD.SolubleALCAM(sALCAM)inducesdivergentBIOLOGicalsignalsandresponses.
ALCAMshowsacharacteristic,temporalandspatialdistributionindevelopmentofawidevarietyoftissuesandcellsinhealthanddisease.ALCAMisexpressedonawidevarietyofcells,withintheleukocytepopulationitsexpressionisparticularlyhighondentriticcells.ALCAMisinvolvedinvariousphysiologicalprocessesincludinghematopoieses,thymusdevelopment,theimmuneresponse,neuriteextension,neuralcellmigration,embryogenesis,neurogenesis,angiogenesis,osteogenesisandisaMarkerforpluripotentmesenchymalstemcells.
ThemonoclonalantibodyAZN-L50completelyblockshomophilicALCAM-ALCAMinteraction.
Specifications:
| Catalognumber | HM2002 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunofluorescence,Immunoprecipitation,Paraffinsections,Westernblot |
| ApplicationNotes | IHC-F:-Tissuesectionswerefixedinacetone FC:AntibodyAZN-L50stainstheextracellulardomainofALCAM.K562cells(2x105)werestainedwith4 µg/mlantibody. FS:AntibodyAZN-L50functionsasanblockingantibodyforhomotypicALCAM-ALCAMinteraction. IF:AntibodyAZN-L50(4 µg/ml)wasusedonmethanol/acetonefixedmonolayersofcellsgrownonaglasssurface. IP:RADIoactivelabelledKG1acellswerelysedandprecipitatedwith1 µgofantibodycoupledtoProteinGSepharose4FastFlowbeads. IHC-P:Tissuesectionswerepretreatedbyheatinginaricesteamerincitratebufferfor10minutesforantigenretrieval.Tissuesectionswereblockedwithnormalhorseserum. W:Anon-reducedsampletreatmentand6,5%SDS-Pagewasused.Thematureproteinhasabandsizeof100kDaandthesolubleform30kDa. |
| Use | Forimmunohistology,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | CD166expressingK562cells |
| Isotype | MouseIgG2a |
| Species | Human |
| Alias | Activatedleukocytecelladhesionmolecule,ALCAM,CD166. |
| References | 1.Kempenvan,Letal;Molecularbasisforthehomophilicactivatedleukocytecelladhesionmolecule(ALCAM)-ALCAMinteraction.JBiolChem2001,276:25783 2.Lunter,Petal;Activatedleukocytecelladhesionmolecule(ALCAM/CD166/MEMD),anovelactorininvasivegrowth,controlsmatrixmetalloproteinaseactivity.CancerRes2005,65:8801 3.Zimmerman,Aetal;Long-termengagementofCD6andALCAMisessentialforT-cellproliferationinducedbydendriticcells.Blood2006,107:3212 4.Riette,Jetal;DistinctkineticandmechanicalpropertiesgovernALCAM-mediatedinteractionsasshownbysingle-moleculeforcespectroscopy.JCellSci2007,120:3965 5.Bladergroen,Betal;SpatiallyseparateddistributionandhighlyflexIBLeexpressionofadhesionmoleculesfacilitatesdynamichematopoiesis.JMedSci2007,7:1239 6.Kilsdonkvan,Jetal;Attenuationofmelanomainvasionbyasecretedvariantofactivatedleukocytecelladhesionmolecule.CancerRes2008,68:3671 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Tumorimmunology |
