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商品描述
Description:GILT,Mouse,mAbMaP.mGILT6
AntibodiesderivedfromcloneMaP.mGILT6recognizemousegamma-interferon-inducIBLelysosomalthiolreductase(GILT).GILTfacilitatesmajorhistocompatibilitycomplex(MHC)classII-restrictedprocessingthroughreductionofproteindisulfidebondsintheendocyticpathway.GILTcanalsofacilitatethetransferofdisulfide-containingantigensintothecytosol,enhancingtheircross-presentationbyMHCclassI.HumanGILTconsistsof261aminoacidswitha37aminoacidsignalsequenceanda224aminoacidprecursorform.Ingeneral,the35kDaprecursoristaggedwithmannose-6-phosphate(M6P)residuesandtransportedtotheendocyticpathwayviatheM6Preceptor(M6PR).Asmallfractionoftheprecursorissecretedasadisulfide-linkeddimer.Inearlyendosomes,N-andC-terminalpro-peptidesarecleavedtogeneratea28kDamatureform.ThematureformofGILTislocalizedtolateendosomesandlysosomesandhasmaximalreductaseactivityattheacidicpHfoundinthesecompartments.GILTisconstitutivelyexpressedinprofessionalantigenpresentingcells(APC).TheconstitutiveexpressionofGILTinAPCsislikelytoaccountforenhancedreductionofproteinsinthelateendosomal,lysosomal,andphagosomalcompartments.AcombinationofGILT-mediatedreductionandproteolysisinthethesecompartmentscanenhancethegenerationofclassIIepitopesaswellasfacilitatethetranslocationofinternalizedantigensintothecytosolforproteosomalprocessingandcross-presentation.Itisalsoconstitutivelyexpressedinthymocytes,matureTcells,andsomefibroblasts.IFN-ɣplaysisthekeyfactorininducingexpressionofMHCclassIIandothercomponentsoftheclassII-restrictedprocessingpathway.IFN-ɣsignalsthroughaspecificcellsurfacereceptorfollowedbyactivationofJAK/STAT1signalling.
Specifications:
Catalognumber | HM1128 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
Application | Flowcytometry,Immunofluorescence,Immunoprecipitation,Westernblot |
Use | ForFlowCytometry,ImmunofluorescenceandImmunoPrecipitation,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions. |
Immunogen | Insect-cellexpressedrecombinantmouseGILT |
Isotype | MouseIgG1 |
Species | Mouse |
Alias | Lfi30,Interferon-gamma-induciblelysosomalreductase |
StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Infectiousdiseases,Tumorimmunology |