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商品描述
Description:EMAP-II,Mouse,mAbM7/1
Monoclonalantibody-cloneM7/1recognizeshumanendothelial-monocyte-activatingpolypeptideII(EMAPII).EMAPIIisaproinflammatorycytokineandchemoattractantofmacrophagesandpolymorphonuclearlymphocytes.TheproteinwasinitiallyidentifiedasaproductofmurinemethylcholanthreneA-inducedfibrosarcomacells.EMAPIIissynthesizedasa34kDaprecursormolecule(proEMAP),-itcanrunanomalouslyinSDSpageat43kDa,andisenzymaticallycleavedtoproduceaBIOLOGicallyactive22kDamaturepolypeptide.Itmodulatesarangeofpropertiesofendothelialcells,monocytes,andneutrophilsinvitro,andinducesanacuteinflammatoryreactionandtumorregressioninvivo.Lateritwasfoundtobeanti-angiogenicandcapableofinductionofapoptosisinproliferationandhypoxicendothelialcellsinvitrointumorvasculatureinvivo.EMAPIIisreleasedfromcells,byayetunknownmechanism,aspro-andmatureEMAPIIproteinsinresponsetovariousformsofcellularstresses,includingglucosestarvationandhypoxia.Endothelialcellsarethemaintarget-forEMAPIIandexplaintheanti-angiogenicproperty.Theanti-tumoreffectisexpectedtoalargeextenttobemediatedthroughthischaracteristic.EMAPIIisalsoassociatedwithbraindiseasesandinjury.EMAPIIexpressionisrelatedwithleukocytes-infiltratedinflamedtissueandsubsequentapoptosis.Insolidtumorsthereisalsoanimmunosuppressiveroleidentified.EMAPIIinducingtumorcellsinduceapoptosisoflymphocytes.EMPAIIisconsideredaMarkerformicroglialcellsandmacrophagesinbraindiseases.ThedistributionofEMAPIIanditsprecursorinbiopsiesmightbeapplicableasadditivediagnostictool.
Specifications:
Catalognumber | HM1124 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
Application | Flowcytometry,Functionalstudies,Immunoassays,Westernblot |
Use | ForimmuneassayandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
Immunogen | Recombinantfull-lengthmouseEMAPII |
Isotype | RabbitIg |
Species | Mouse |
Alias | Endothelialcellandmonocyteactivatingproinflammatorycytokine |
References | 1.Rajashekhar,Getal;Amonoclonalratanti-mouseEMAPIIantibodythatfunctionallyneutralizespro-andmature-EMAPIIinvitro.JImmunolMethods2009,350:22. 2.Clauss,Metal;Lungendothelialmonocyte-activatingprotein2isamediatorofcigarettesmoke-inducedemphysemainmice.JClinInvest2011,121:2470. |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Tumorimmunology |