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苏州工业园区生物纳米园A4#216
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商品描述
Description:CD55,Mouse,mAb3D5,FITC
Themonoclonalantibody3D5recognizescomplementdecayacceleratingfactor(DAF),alsodesignatedasCD55.Cellsexpressontheirsurfaceseveralproteinswhichprotectagainstcomplementattack,namelyCreceptorI(CR1),decayacceleratingfactor,membranecofactorprotein(MCP)andCD59.CR1,CD55andMCPregulatetheactivationpathwaysofcomplementbyeitheracceleratingdecayoftheC3andC5convertase(CR1,CD55),oractingascofactorsfortheserineproteasefactorI,whichcleavesandirreversIBLyinactivatesC3b(CR1,MCP).
MouseCD55isa60kDatransmembraneproteinthatbindsC3bandC4btoinhibitformationandhalf-lifeoftheC3convertases.CD55isbroadlydistributedamongcellsincontactwithserum,includingbothhaematopoieticandnonhaematopoieticcells.AlthoughCD55doesnothaveanessentialroleincontrollinghemolysisoferythrocytes,ithasanimportantroleinregulationofthedepositionofC3onnucleatedcells.
TogetherwithothercomplementregulatorsCD55protectsself-cellsfromautologouscomplement-mediatedinjury.CD55cooperateswithCD46incircumventingautologousC3deposition,whileCD59inhibitsthepathwayatthecriticalend-point.
MouseCD55isa60kDatransmembraneproteinthatbindsC3bandC4btoinhibitformationandhalf-lifeoftheC3convertases.CD55isbroadlydistributedamongcellsincontactwithserum,includingbothhaematopoieticandnonhaematopoieticcells.AlthoughCD55doesnothaveanessentialroleincontrollinghemolysisoferythrocytes,ithasanimportantroleinregulationofthedepositionofC3onnucleatedcells.
TogetherwithothercomplementregulatorsCD55protectsself-cellsfromautologouscomplement-mediatedinjury.CD55cooperateswithCD46incircumventingautologousC3deposition,whileCD59inhibitsthepathwayatthecriticalend-point.
Specifications:
Catalognumber | HM1115F |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100μg/ml)0.2μmproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1%bovineserumalbuminand0.02%sodiumazide. |
Conjugate | FITC |
Application | Flowcytometry,Immunoassays,Paraffinsections,Westernblot |
ApplicationNotes | IA:HM1115wasusedasadetectionantibodyinadirectELISA(platescoatedwithmouseCD55.TheconcentrationofHM1115usedwas2μg/ml. W:ProteinswereseparatedbySDS-PAGE,transferredtonitrocellulosemembranesandblockedwithTris-bufferedsalinecontaining0.05%Tween20(TBST),3%non-fatdriedmilkand3%BSA(Ref1). P:Amixtureof3D5andanotherantibodywasused.Colonsegmentswerefixedovernightin10%Formalin,embeddedinparaffinblocks,andcutinto5μmsections.SectionswerestainedwithH&E(Ref.5). |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Immunogen | NRKcellsexpressingtransmembrane-anchoredmouseDAF |
Isotype | RatIgG2a |
Species | Mouse |
Alias | Complementdecay-acceleratingfactor,GPI-anchored,DAF-GPI Genename:Cd55,Cd55a,Daf,Daf1 |
References | 1.Spiller,Oetal;Efficientgenerationofmonoclonalantibodiesagainstsurface-expressedproteinsbyhyperexpressioninrodentcells.JImmunolMeth1999,224:51 2.Harris,Cetal;Humanandrodentdecay-acceleratingfactors(CD55)arenotspeciesrestrictedintheircomplement-inhibitingactivities.Immunol2000,100:462 3.Lin,Fetal;Tissuedistributionofproductsofthemousedecay-acceleratingfactor(DAF)genes.ExploitationofaDaf1knock-outmouseandsite-specificmonoclonalantibodies.Immunol2001,104:215 4.Spiller,Oetal;EchovirusesandcoxsackieBvirusesthatusehumandecay-acceleratingfactor(DAF)asareceptordonotbindtherodentanaloguesofDAF.JofInfectDis2000,181:340 5.Lin,Fetal;Decay-AcceleratingFactorDeficiencyIncreasesSusceptibilitytoDextranSulfateSodium-InducedColitis:RoleforComplementinInflammatoryBowelDisease.JImmunol2004,172:3836 6.Kinderlerer,Aetal;Hemeoxygenase-1expressionenhancesvascularendothelialresistancetocomplementmediatedinjurythroughinductionofdecay-acceleratingfactor.Aroleforincreasedbilirubinandferritin.Blood2008 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Autoimmunity,Infectiousdiseases |