品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description:CD155,Mouse,mAb3F1
Themonoclonalantibody3F1recognizesmousepoliovirusreceptor,alsonamedCD155.CD155isexpressedoncellsofhematopoieticoriginandbyendothelialvesselsinsidesecondarylymphoidorgansincludingHEV.Uponactivation,dendriticcells,TandBcellsupregulatethesurfacelevelofCD155.CD155isan~45kDamemberofasubfamilyofimmunoglobulin-likeadhesionreceptors.Nectin1-4areothermembersofthissubfamily,functioningincell-cellcontactbyformingheterophilicandhemophilictrans-dimers.Incontrasttonectins,CD155lacksselfadhesionpotentialbutcaninteractwithothernectins.Thisway,CD155contributesintheformationofadherensjunctionsbetweenendothelialandepithelialcells.Duetothisadhesivecapacity,CD155influencesprocessessuchascellmotility,migration,growth,andformationofadherensjunctions.FurThermore,CD155isinvolvedinimmunologicalprocessessuchasCD8Tcell-mediatedtumorrejection,andNKcell-mediatedkilling.Manytumortypesdisplayanaberrantup-regulationofCD155makingthemvulnerabletodestructionbyNKorcytotoxicTcells.
Inhuman,poliovirus,vitronectinandseveralothercellularreceptorswereidentifiedasligandsthatbindtoCD155.CD96(TACTILE)andCD226(DNAM-1),bothmembersoftheIg-superfamily,areotherligandsofhumanCD155.MurineCD155doesnotserveasafunctionalpoliovirusreceptor,however,murineCD155recognizesthesamepanelofligandslikenectin-3,CD96,CD226andvitronectin.CD155-deficientmicedevelopnormally,however,areducedhumoralimmuneresponsetoantigensenteringtheorganismviathegastrointestinaltractisshown.
Inhuman,poliovirus,vitronectinandseveralothercellularreceptorswereidentifiedasligandsthatbindtoCD155.CD96(TACTILE)andCD226(DNAM-1),bothmembersoftheIg-superfamily,areotherligandsofhumanCD155.MurineCD155doesnotserveasafunctionalpoliovirusreceptor,however,murineCD155recognizesthesamepanelofligandslikenectin-3,CD96,CD226andvitronectin.CD155-deficientmicedevelopnormally,however,areducedhumoralimmuneresponsetoantigensenteringtheorganismviathegastrointestinaltractisshown.
Specifications:
Catalognumber | HM1106 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
Application | Flowcytometry,Functionalstudies |
ApplicationNotes | FC:Antibodyclone3F1stainstheextracellulardomainofmouseCD155.Thethymocyteswereblockedwith5%mouseserumbeforeincubationwithclone3F1for1hourat4 °CAspositivecontrol-CD4+CD8+thymocytes-wasusedandasnegativecontrolCD155-/-derivedcells(Ref.1). FS:Antibodyclone3F1functionsasantagoNIST.TheantibodywasfunctionallytestedbyblockingadhesionofSf9cellsexpressingmCD155toRMA,CD4+andCD8+cells.Furthermore,antibodyclone3F1prevenstformationofmCD155/ligandcomplexes(Ref.2). |
Use | Forimmunoassays,immunoprecipitation,immunohistology,flowcytometryandfunctionalstudiesdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Immunogen | EctodomainofrecombinantHis-taggedCD155 |
Isotype | RatIgG2a |
Species | Mouse |
Alias | Poliovirusreceptor,Pvr,nectin-likeprotein5,Tage4 |
PositiveControl | CD4+CD8+mousethymocytes |
References | 1.Maier,M.etal;TheadhesionreceptorCD155determinesthemagnitudeofhumoralimmuneresponsesagainstorallyingestedantigens.EurJImmunol2007,37:2214 2.Seth,Setal;ThemurinepanTcellMarkerCD96isanadhesionreceptorforCD155andnectin-1.BiochemBiophysResCommun.2007,364:959 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
Disease | Tumorimmunology |