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商品描述
Description:CD200R3,Mouse,mAbBa91,FITC
ThemonoclonalantibodyBa91recognizesmouseCD200Receptor-like3(CD200R3),aproteinof~38kDaor~74kDa(reducedornon-reducedcondition,respectively).CD200R3presumablyexistsasadisulfide-linkeddimeronthecellsurface.
TheOX-2,orCD200receptor(CD200R),belongstotheimmunoglobulinsuperfamily.CD200RcontainstwoIg-likeextracellulardomainsandmediatesinhibitorysignalsinmyeloidcells.MicedeficientfortheligandCD200developenhancedexperimentalallergicencephalomyelitisandcollagen-inducedarthritis.UnlikeCD200R,theCD200R-likereceptorsfunctionsasactivatingreceptors.TheycontainshortcytoplasmictailsandalysineresidueinthetransmembraneregionandarelikelytosignalviaadaptorproteinssuchasDAP12,DAP10,FcRγ,orCD3ζ.
CD200R3isexpressedpreferentiallyoncellsofthemyeloidlineage,includingmastcellsandbasophils.Bothcelltypesareinvolvedinthehostdefensesystemagainstpathogensandinthedevelopmentofallergicdisorders.Theyfunctionasindependentessentialinitiatorsofallergicreactions.Basophilsaretheleastcommonleukocytesintheperipheralbloodaccountingapproximately0.5%ofallleukocytes.InvivodepletionofbasophilsbyusingmonoclonalantibodyprovedtheessentialroleofbasophilsinthedevelopmentandmaintenanceofIgE-mediatedchronicallergicinflammation.
TheactivatingCD200receptors,likeCD200R3,playanimportantroleinIgE-independentmastcellandbasophilactivation.MonoclonalantibodyBa91activatesbasophilsexvivoandelicitssystemicanaphylaxiswhenadmiNISTeredinvivo.Cross-linkingofCD200R3onMC/9cellsbymonoclonalantibodyBa91inducesdegranulation.MonoclonalantibodyBa91isalsousefulfordepletionofbasophils.
TheOX-2,orCD200receptor(CD200R),belongstotheimmunoglobulinsuperfamily.CD200RcontainstwoIg-likeextracellulardomainsandmediatesinhibitorysignalsinmyeloidcells.MicedeficientfortheligandCD200developenhancedexperimentalallergicencephalomyelitisandcollagen-inducedarthritis.UnlikeCD200R,theCD200R-likereceptorsfunctionsasactivatingreceptors.TheycontainshortcytoplasmictailsandalysineresidueinthetransmembraneregionandarelikelytosignalviaadaptorproteinssuchasDAP12,DAP10,FcRγ,orCD3ζ.
CD200R3isexpressedpreferentiallyoncellsofthemyeloidlineage,includingmastcellsandbasophils.Bothcelltypesareinvolvedinthehostdefensesystemagainstpathogensandinthedevelopmentofallergicdisorders.Theyfunctionasindependentessentialinitiatorsofallergicreactions.Basophilsaretheleastcommonleukocytesintheperipheralbloodaccountingapproximately0.5%ofallleukocytes.InvivodepletionofbasophilsbyusingmonoclonalantibodyprovedtheessentialroleofbasophilsinthedevelopmentandmaintenanceofIgE-mediatedchronicallergicinflammation.
TheactivatingCD200receptors,likeCD200R3,playanimportantroleinIgE-independentmastcellandbasophilactivation.MonoclonalantibodyBa91activatesbasophilsexvivoandelicitssystemicanaphylaxiswhenadmiNISTeredinvivo.Cross-linkingofCD200R3onMC/9cellsbymonoclonalantibodyBa91inducesdegranulation.MonoclonalantibodyBa91isalsousefulfordepletionofbasophils.
Specifications:
Catalognumber | HM1104F |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1%bovineserumalbuminand0.02%sodiumazide. |
Conjugate | FITC |
Application | Flowcytometry,Functionalstudies,Immunoprecipitation |
ApplicationNotes | FC:MC/9cellswerewashed,resUSPendedinFACSbuffer(PBSwith2%BSA),andincubatedfor30minwithHM1104. |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Immunogen | Mouseprimarybasophils |
Isotype | RatIgG2a |
Species | Mouse |
Alias | CellsurfaceglycoproteinCD200receptor3,CD200receptor-like3,CD200cellsurfaceglycoproteinreceptor-likeb,CD200RLb,cellsurfaceglycoproteinOX-2receptor3 Genename:Cd200r3,Cd200rlb |
PositiveControl | MC/9 |
NegativeControl | CFTL-15cells,T-cells,B-cells,macrophages,neutrophils |
References | 1.Kojima,Tetal;MastcellsandbasophilsareselectivelyactivatedinvitroandinvivothroughCD200R3inanIgE-independentmanner.JImmunol2007,179:7093 2.Sullivan,Betal;Basophils:ANonredundantContributortoHostImmunity.Immunityreview2008,30:12 3.DeHaan,Jetal;Lipid-richenteralnutritionregulatesmucosalmastcellactivationviathevagalanti-inflammatoryreflex.AmJPhysiolGastLiverPhysiol,305:G383 4.Yang,Yetal;BasophilactivationthroughASGM1stimulationtriggersPAFreleaseandanaphylaxis-likeshockinmice.EurJImmunol2014,44:2468 5.Nakamura,Tetal;SelectivedepletionofbasophilsamelioratesimmunoglobulinE-mediatedanaphylaxis.BiochemBiophReports2017,9:29 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
Disease | Autoimmunity |