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商品描述
Description:CD200R3,Mouse,mAbBa103,FITC
ThemonoclonalantibodyBa103recognizesmouseCD200Receptor-like3(CD200R3),aproteinof~38kDaor~74kDa(reducedornon-reducedcondition,respectively).CD200R3presumablyexistsasadisulfide-linkeddimeronthecellsurface,itisasingle-passtypeImembraneprotein.
TheOX-2,orCD200receptor(CD200R),belongstotheimmunoglobulinsuperfamily.CD200RcontainstwoIg-likeextracellulardomains,oneIg-likeC2-type(immunoglobulin-like)domainandoneIg-likeV-type(immunoglobulin-like)domain.andmediatesinhibitorysignalsinmyeloidcells.MicedeficientfortheligandCD200developenhancedexperimentalallergicencephalomyelitisandcollagen-inducedarthritis.UnlikeCD200R,theCD200R-likereceptorsfunctionasactivatingreceptors.TheycontainshortcytoplasmictailsandalysineresidueinthetransmembraneregionandarelikelytosignalviaadaptorproteinssuchasDAP12,DAP10,FcRγ,orCD3ζ.
CD200R3isexpressedinuterusandbonemarrow-derivedmastcells(atproteinlevel).Itisexpressedinuterus,spleen,bonemarrow-deriveddendritic,basophilandmastcells.CD200R3isalsoexpressedinthelungofN.brasiliensis-infectedmice.Itisweaklyexpressedinbrain,testis,lungandthymus.
CD200R3isexpressedpreferentiallyoncellsofthemyeloidlineage,includingmastcellsandbasophils.Bothcelltypesareinvolvedinthehostdefensesystemagainstpathogensandinthedevelopmentofallergicdisorders.Theyfunctionasindependentessentialinitiatorsofallergicreactions.Basophilsaretheleastcommonleukocytesintheperipheralbloodaccountingapproximately0.5%ofallleukocytes.InvivodepletionofbasophilsbyusingmonoclonalantibodyBa103provedtheessentialroleofbasophilsinthedevelopmentandmaintenanceofIgE-mediatedchronicallergicinflammation.
TheactivatingCD200receptors,likeCD200R3,playanimportantroleinIgE-independentmastcellandbasophilactivation.MonoclonalantibodyBa103activatesbasophilsexvivoandelicitssystemicanaphylaxiswhenadmiNISTeredinvivo.Cross-linkingofCD200R3onMC/9cellsbymonoclonalantibodyBa103inducesdegranulation.FurThermore,monoclonalantibodyBa103isusefulfordepletionofbasophils.Thisantibodydoesnotcross-reactwithCD200R,CD200R2,CD200R4orCD200R5.
TheOX-2,orCD200receptor(CD200R),belongstotheimmunoglobulinsuperfamily.CD200RcontainstwoIg-likeextracellulardomains,oneIg-likeC2-type(immunoglobulin-like)domainandoneIg-likeV-type(immunoglobulin-like)domain.andmediatesinhibitorysignalsinmyeloidcells.MicedeficientfortheligandCD200developenhancedexperimentalallergicencephalomyelitisandcollagen-inducedarthritis.UnlikeCD200R,theCD200R-likereceptorsfunctionasactivatingreceptors.TheycontainshortcytoplasmictailsandalysineresidueinthetransmembraneregionandarelikelytosignalviaadaptorproteinssuchasDAP12,DAP10,FcRγ,orCD3ζ.
CD200R3isexpressedinuterusandbonemarrow-derivedmastcells(atproteinlevel).Itisexpressedinuterus,spleen,bonemarrow-deriveddendritic,basophilandmastcells.CD200R3isalsoexpressedinthelungofN.brasiliensis-infectedmice.Itisweaklyexpressedinbrain,testis,lungandthymus.
CD200R3isexpressedpreferentiallyoncellsofthemyeloidlineage,includingmastcellsandbasophils.Bothcelltypesareinvolvedinthehostdefensesystemagainstpathogensandinthedevelopmentofallergicdisorders.Theyfunctionasindependentessentialinitiatorsofallergicreactions.Basophilsaretheleastcommonleukocytesintheperipheralbloodaccountingapproximately0.5%ofallleukocytes.InvivodepletionofbasophilsbyusingmonoclonalantibodyBa103provedtheessentialroleofbasophilsinthedevelopmentandmaintenanceofIgE-mediatedchronicallergicinflammation.
TheactivatingCD200receptors,likeCD200R3,playanimportantroleinIgE-independentmastcellandbasophilactivation.MonoclonalantibodyBa103activatesbasophilsexvivoandelicitssystemicanaphylaxiswhenadmiNISTeredinvivo.Cross-linkingofCD200R3onMC/9cellsbymonoclonalantibodyBa103inducesdegranulation.FurThermore,monoclonalantibodyBa103isusefulfordepletionofbasophils.Thisantibodydoesnotcross-reactwithCD200R,CD200R2,CD200R4orCD200R5.
Specifications:
| Catalognumber | HM1103F |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | FITC |
| Application | Flowcytometry,Functionalstudies,Immunoprecipitation,Westernblot |
| ApplicationNotes | WB:reducedandnon-reducedMC/9lysate;bandsize38kDaunderreducingconditionsand74kDaundernonreducingconditions FC:Basophilsandmastcells;nofixationneeded FS:antagonistinbasophil-mediatedallergicreactions;asingleintravenousadministrationof30μgantibody.TheBIOLOGicalactivityoftheantibodyisdrasticreductioninthebasophilenumberintheperipheralbloodandspleen,toapproximately20%ofthenormallevel;alsousefulformastcelldegranulation;readoutbymeasuringhexosaminidaseactivity IP:mastcellswerelysedwith1%NP-40containinglysisbufferwithproteinaseinhibitormixturebeforeincubationwiththeantibody |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Mouseprimarybasophils;cell-enrichedbonemarrowcells |
| Isotype | RatIgG2b |
| Species | Mouse |
| Alias | CellsurfaceglycoproteinCD200receptor3,CD200receptor-like3,CD200cellsurfaceglycoproteinreceptor-likeb,CD200RLb,cellsurfaceglycoproteinOX-2receptor3 Genename:Cd200r3,Cd200rlb |
| PositiveControl | MC/9cells |
| NegativeControl | CFTL-15cells,T-cells,B-cells,macrophages,neutrophils |
| References | 1.Obata,Ketal;Basophilsareessentialinitiatorsofanoveltypeofchronicallergicinflammation.Blood2007,110:913 2.Kojima,Tetal;MastcellsandbasophilsareselectivelyactivatedinvitroandinvivothroughCD200R3inanIgE-independentmanner.JImmunol2007,179:7093 3.Khodoun,Metal;Peanutscancontributetoanaphylacticshockbyactivatingcomplement.JAllergyClinImmunol2009,123:342 4.Galli,Setal;BasophilsAreBack!Immunitypreviews2008,28:495 5.Mukai,Ketal;NewInsightsintotheRolesforBasophilsinAcuteandChronicAllergy.AllerInt2009,58:11 6.Weinberger,Eetal;Generationofhypoallergenicneoglycoconjugatesfordendriticcelltargetedvaccination:Anoveltoolforspecificimmunotherapy.JControlRelease2013,165:101 7.Han,Hetal;Thymicstromallymphopoietin–mediatedepicutaneousinflammationpromotesacutediarrheaandanaphylaxis.JClinInves2014,124:5442 8.Yang,Yetal;BasophilactivationthroughASGM1stimulationtriggersPAFreleaseandanaphylaxis-likeshockinmice.EurJImmunol2014,44:2468 9.Otsuka,Aetal;Mastcellsandbasophilsincutaneousimmuneresponses.EurJAllergy2015,70:131 10.Beutier,Hetal;IgGsubclassesdeterminepathwaysofanaphylaxisinmice.JAllClinImmunol2017,139:269 11.Miyaki,Ketal;Emergingrolesofbasophilsinallergicinflammation.AllerInt2017,66:382 12.Nakamura,Tetal;SelectivedepletionofbasophilsamelioratesimmunoglobulinE-mediatedanaphylaxis.BiochemBiophReports2017,9:29 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
| Disease | Autoimmunity |
