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商品描述
Description:CD200R3,Mouse,mAbBa103
ThemonoclonalantibodyBa103recognizesmouseCD200Receptor-like3(CD200R3),aproteinof~38kDaor~74kDa(reducedornon-reducedcondition,respectively).CD200R3presumablyexistsasadisulfide-linkeddimeronthecellsurface,itisasingle-passtypeImembraneprotein.TheOX-2,orCD200receptor(CD200R),belongstotheimmunoglobulinsuperfamily.CD200RcontainstwoIg-likeextracellulardomains,oneIg-likeC2-type(immunoglobulin-like)domainandoneIg-likeV-type(immunoglobulin-like)domain.andmediatesinhibitorysignalsinmyeloidcells.MicedeficientfortheligandCD200developenhancedexperimentalallergicencephalomyelitisandcollagen-inducedarthritis.UnlikeCD200R,theCD200R-likereceptorsfunctionasactivatingreceptors.TheycontainshortcytoplasmictailsandalysineresidueinthetransmembraneregionandarelikelytosignalviaadaptorproteinssuchasDAP12,DAP10,FcRg,orCD3z.CD200R3isexpressedinuterusandbonemarrow-derivedmastcells(atproteinlevel).Itisexpressedinuterus,spleen,bonemarrow-deriveddendritic,basophilandmastcells.CD200R3isalsoexpressedinthelungofN.brasiliensis-infectedmice.Itisweaklyexpressedinbrain,testis,lungandthymus.CD200R3isexpressedpreferentiallyoncellsofthemyeloidlineage,includingmastcellsandbasophils.Bothcelltypesareinvolvedinthehostdefensesystemagainstpathogensandinthedevelopmentofallergicdisorders.Theyfunctionasindependentessentialinitiatorsofallergicreactions.Basophilsaretheleastcommonleukocytesintheperipheralbloodaccountingapproximately0.5%ofallleukocytes.InvivodepletionofbasophilsbyusingmonoclonalantibodyBa103provedtheessentialroleofbasophilsinthedevelopmentandmaintenanceofIgE-mediatedchronicallergicinflammation.TheactivatingCD200receptors,likeCD200R3,playanimportantroleinIgE-independentmastcellandbasophilactivation.MonoclonalantibodyBa103activatesbasophilsexvivoandelicitssystemicanaphylaxiswhenadmiNISTeredinvivo.Cross-linkingofCD200R3onMC/9cellsbymonoclonalantibodyBa103inducesdegranulation.FurThermore,monoclonalantibodyBa103isusefulfordepletionofbasophils.
Specifications:
| Catalognumber | HM1103 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies,Immunoprecipitation,Westernblot |
| ApplicationNotes | WB:reducedandnon-reducedMC/9lysate;bandsize38kDaunderreducingconditionsand74kDaundernonreducingconditions FC:Basophilsandmastcells;nofixationneeded FS:antagonistinbasophil-mediatedallergicreactions;asingleintravenousadministrationof30 µmantibody.TheBIOLOGicalactivityoftheantibodyisdrasticreductioninthebasophilenumberintheperipheralbloodandspleen,toapproximately20%ofthenormallevel;alsousefulformastcelldegranulation;readoutbymeasuringhexosaminidaseactivity IP:mastcellswerelysedwith1%NP-40containinglysisbufferwithproteinaseinhibitormixturebeforeincubationwiththeantibody |
| Use | Forflowcytometry,Westernblottingandimmunoprecipitation,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsetting. |
| Immunogen | Mouseprimarybasophils;cell-enrichedbonemarrowcells |
| Isotype | RatIgG2b |
| Species | Mouse |
| Alias | CD200R3,CD200RLb,OX-2Receptor3 |
| PositiveControl | MC/9cells |
| References | 1.Obata,Ketal;Basophilsareessentialinitiatorsofanoveltypeofchronicallergicinflammation.Blood2007,110:913 2.Kojima,Tetal;MastcellsandbasophilsareselectivelyactivatedinvitroandinvivothroughCD200R3inanIgE-independentmanner.JImmunol2007,179:7093 3.Khodoun,Metal;Peanutscancontributetoanaphylacticshockbyactivatingcomplement.JAllergyClinImmunol2009,123:342 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
| Disease | Autoimmunity |
