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商品描述
Description:EphrinB4,Mouse,mAbVEB4-7E4
ThemonoclonalantibodyVEB4-7E4recognizesmouseEphrintype-Breceptor4(EphB4),an~110kDprotein.Erythropoietin-producinghumanhepatocellularcarcinoma(Eph)receptorsandephrinsaremembraneproteins.Theyareclassifiedinto2broadsubclasses,namelyAandB,accordingtostructuralhomologiesandbindingspecificities.Ephreceptorsaretyrosinekinases,whichautophosphorylateuponbindingtotheircognateephrinligands.Ephreceptorsandephrinsarefrequentlyexpressedinreciprocalpatternsthatcorrelatewithcellularboundariesduringembryonicdevelopment.TheinteractionbetweenEphB4anditsligand,Ephrin-B2,playsanimportantroleincell-cell,cell-extracellularmatrixinteractionsaswellasincellmigration,adhesionandproliferation.Duringtheearlystagesofvasculardevelopment,EphB4isspecificallyexpressedinvenousendothelium,whereasEphrin-B2isexpressedinarterialendothelium.Inmouseembryo"s,EphB4andEphrin-B2areessentialforembryonicheartdevelopmentandangiogenesis.Inadultmicrocirculation,EphB4isnotaubiquitousMarkerofarterial/venouspolarity,butisexpressedalongbothvenulesandarterioles.FurThermore,EphB4isupregulatedbyendothelialcellsalongblind-endedcapillarysproutsversusconnectingcapillaries.Assuch,EphB4isthoughttoplayaroleinthepatterningofnewvesselsduringangiogenesis.EphB4isalsoexpressedinavarietyoftumorcells,likegastrointestinal,prostate,bladder,breast,liver,lungandovariancancers,aswellasleukemia,mesothelioma,malignantbreasttumorsandmelanoma.ReductionofEphB4activityacceleratedtumOrigenesisincolonandrectum.Inheadandnecksquamouscellcarcinomaandendometrialcarcinoma,overexpressionofEphB4isinverselyrelatedtoapoorprognosis.However,inmesothelioma,up-regulationofEphB4resultedingrowthofthetumor.BesidestheessentialexpressionofEphB4,coexpressionofotherEphB4familymembersorEphB-ligandsmayaffecttumorcellviABIlityandproliferationaswell.ThemonoclonalantibodyVEB4-7E4marksvenousendothelialcells,butnotarterialendothelialcellsinB16melanomacells.
Specifications:
Catalognumber | HM1099 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100 µg/ml)0.2 µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
Application | Flowcytometry,Frozensections,Immunoassays,Immunofluorescence,Westernblot |
Use | Forimmunohistology,flowcytometry,immunofluorescenceandWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Isotype | RatIgG2a |
Species | Mouse |
Alias | Tyrosine-proteinkinasereceptorHTK |
References | Huang,Xetal;EphB4overexpressioninB16melanomacellsaffectsarterial-venouspatterningintumorangiogenesis.CancerRes2007,67:9800 |
Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
Disease | Neurologicaldisorders,Tumorimmunology |