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商品描述
Description:Fibroblasts,Mouse,mAbER-TR7
MonoclonalantibodyER-TR7recognizeswithanintracellularcomponentofmousefibroblasts.Fibroblastsaretheleastspecializedcellsintheconnective-tissuefamily.Theyaredispersedinconnectivetissuethroughoutthebody,wheretheysecreteanonrigidextracellularmatrix(ECM)thatisrichintypeIand/ortypeIIIcollagen.Conectivetissueconsistsofglycosaminoglycans,proteoglycansandglycoproteinsthroughwhichvariousfibresrun.Thesefibrescanbecollagenous,elasticorreticular.Reticularfibresarecomposedfromthefamilyofcollagenproteinsandgivetensilestrength.Thesefibresaremadebyreticularfibroblasts.Theactivationoffibroblastsbyinflammatorystimuliresultsintheirmigration,proliferationanddepositionofextracellularmatrixcomponents,importantfeaturesinvolvedinbothwoundhealingandfibrosis.
TheER-TR7antigenisaubiquitouscomponentofstromal(interstitial)matrixcartilageandofatleastsomebasementmembranezones.Theantigendetectedisnotabasementmembranecomponent,noranymajorcollagentypeorfibronectin.Theantigendetectedhasawidertissuedistributionthanreticulin.ER-TR7detectsanintracellularcomponentoffibroblasts.SinceER-TR7doesnotreactwithpurifiedlaminin,collagentypesI-V,fibronectin,heparinsulfateproteoglycan,entactinornidogen,itdetectsahithertouncharacterizedantigen.
ThemonoclonalantibodyER-TR7canbeusedtostudythemicro-anatomyofvariousorgans.ER-TR7outlinesthevariouscompartmentsofperipherallymphoidorgansbycharacteristiclabelingpatterns(nosuchcompartmentsarefoundincentrallymphoidorgans).FurThermoreER-TR7delineatesvarioustypesofconnectivetissuecompartmentsinnonlymphoidorgans.
TheantibodyER-TR7detectsreticularfibroblasts,whichconstitutethecellularframeworkoflymphoidandnonlymphoidorgansandtheirproducts.ER-TR7isusefultoclearlydelineatedthefollicles,periarteriolarlymphoidsheathandmarginalzone;themajorwhitepulpcompartments.Furthermoreinlymphnodes,thecapsule,sinuses,follicles,paracortexandmedullarycordsareclearlydelineated.
TheER-TR7antigenisaubiquitouscomponentofstromal(interstitial)matrixcartilageandofatleastsomebasementmembranezones.Theantigendetectedisnotabasementmembranecomponent,noranymajorcollagentypeorfibronectin.Theantigendetectedhasawidertissuedistributionthanreticulin.ER-TR7detectsanintracellularcomponentoffibroblasts.SinceER-TR7doesnotreactwithpurifiedlaminin,collagentypesI-V,fibronectin,heparinsulfateproteoglycan,entactinornidogen,itdetectsahithertouncharacterizedantigen.
ThemonoclonalantibodyER-TR7canbeusedtostudythemicro-anatomyofvariousorgans.ER-TR7outlinesthevariouscompartmentsofperipherallymphoidorgansbycharacteristiclabelingpatterns(nosuchcompartmentsarefoundincentrallymphoidorgans).FurThermoreER-TR7delineatesvarioustypesofconnectivetissuecompartmentsinnonlymphoidorgans.
TheantibodyER-TR7detectsreticularfibroblasts,whichconstitutethecellularframeworkoflymphoidandnonlymphoidorgansandtheirproducts.ER-TR7isusefultoclearlydelineatedthefollicles,periarteriolarlymphoidsheathandmarginalzone;themajorwhitepulpcompartments.Furthermoreinlymphnodes,thecapsule,sinuses,follicles,paracortexandmedullarycordsareclearlydelineated.
Specifications:
Catalognumber | HM1086 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
Application | Flowcytometry,Frozensections,Immunofluorescence,Paraffinsections |
ApplicationNotes | F:Sectionswerestainedusinganindirectimmunoperoxidasemethod(Ref.1). FC:SplenocyteswereincubatedwithER-TR7for30"(Ref.4). IF:AcetoneorPFAfixedcellswerequenchedwith50mMNH4Clfor30",blockedandpermeABIlizedwith1.5%goatserum/0.1%saponininPBSfor45"atRT.IncubationofER-TR-7inblockandpermsolutionfor45"atRT.Specificstainingwasdetectedwithafluorescentconjugatedoatanti-ratIgG(Ref.5). P:Formalinfixedparaffinsectionsweredeparafined,hydratedinethanolandstainedwithER-TR7for3";atRT(Ref.3). |
Use | Forimmunohistologyandflowcytometrydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:20. |
Immunogen | Mousethymicstromalcells |
Isotype | RatIgG2a |
Species | Rat |
PositiveControl | Spleen |
References | 1.Vlietvan,Eetal;Monoclonalantibodiestostromalcelltypesofthemousethymus.EurJImmunol1984,14:524 2.Vlietvan,Eetal;Reticularfibroblastsinperipherallymphoidorgansidentifiedbyamonoclonalantibody.JHistochemCytochem1986,34:883 3.Kalled,Setal;Anti-CD40ligandantibodytreatmentofSNF1micewithestablishednephritits:preservationofkidneyfunction.JImmunol1998,120:2158 4.Nolte,Metal;Aconduitsystemdistributeschemokinesandsmallblood-bornemoleculesthroughthesplenicwhitepulp.JExpMed2003,3:505 5.Svensson,Metal;Stromalcellsdirectlocaldifferentiationofregulatorydendriticcells.Immunity2004,21:505 6.Alba,Retal;BiodistributionandretargetingofFX-bindingablatedadenovirusSEROtype5vectors.Blood2010(Epubjuly7). |
Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Tumorimmunology |