品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description:CD71,Mouse,mAbER-MP21,FITC
ThemonoclonalantibodyER-MP21recognizesCD71,thetransferrinreceptor1.CD71isa200kDaglycoproteincomposedoftwoidentical,disulfide-linkedchains.Eachchainiscapableofbindingtransferrin,aniron-transportmolecule.Bindingoftransferrintoitsreceptorfollowedbyendocytosisofthereceptor-ligandcomplexisamajorcellulariron-uptakemechanism.Ironuptakebytheproliferatingcellisessentialfortheiron-containingenzymeribonucleotidereductaseinvolvedwithDNAsynthesis.CD71isnotonlyexpressedbycyclingcells,butalsobycellsthatrequireironforotheriron-dependentproteins,suchastheenzymeperoxidaseinmonocytes.Rapidlyproliferatingcells,asinmalignancy,generallyexpressCD71abundantly.FurThermore,CD71hasbeendescribedasaMarkerofimmature,proliferatingTcells.
ThemonoclonalantibodyER-MP21inhibitsproliferationanddifferentiationduringearlyTcelldevelopment.ER-MP21recognizesmurineCD71,butdoesnotcompetewithtransferrinbinding.
ThemonoclonalantibodyER-MP21inhibitsproliferationanddifferentiationduringearlyTcelldevelopment.ER-MP21recognizesmurineCD71,butdoesnotcompetewithtransferrinbinding.
Specifications:
| Catalognumber | HM1085F |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1%bovineserumalbuminand0.02%sodiumazide. |
| Conjugate | FITC |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunoprecipitation |
| ApplicationNotes | FS:AntibodyER-MP21canbeusedforinhibitionofBIOLOGicalactivity.Theantibodyinhibitstheironuptakeandtheproliferationofmacrophageprecursorsfrombonemarrowandearlymacrophageprecursorcelllines. IHC-F:Cryostatsectionswerethaw-mountedonglassslides,fixedfor5mininmethanolpreincubatedwith10%goatserumandincubatedatroomtemperaturewithrat-anti-mousetransferrinreceptorcloneER-MP21. |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | Mousemacrophagecelllines(Ref.1). |
| Isotype | RatIgG2a |
| Species | Mouse |
| Alias | Transferrinreceptorprotein1,TR,TfR,TfR1,Trfr Genename:Tfrc,Trfr |
| References | 1.Leenen,Petal;Differentialinhibitionofmacrophageproliferationbyanti-transferrinreceptorantibodyER-MP21:correlationtomacrophagedifferentiationstage.ExpCellRes1990,189:55 2.Brekelmans,Petal;Transferrinreceptorexpressionasamarkerofimmaturecyclingthymocytesinthemouse.CellImmunol1994,159:331 3.Brekelmans,Petal;InhibitionofproliferationanddifferentiationduringearlyTcelldevelopmentbyanti-transferrinreceptorantibody.EurJImmunol1994,24:2896 4.Nikolic,Tetal;Developmentalstagesofmyeloiddendriticcellsinmousebonemarrow.IntImmunol2003,15:515 5.Nikolic,Tetal;BoneMarrowPrecursorsofNonobeseDiabeticMiceDevelopintoDefectiveMacrophage-LikeDendriticCellsInVitro.JImmunol2004,173:4342 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity |
