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商品描述
Description:C5aR,Mouse,mAb20/70
Themonoclonalantibody20/70recognizesthemousereceptorforC5a(CD88).ThemouseanaphylatoxincomplementfactorC5a,asplitproductofC5,isoneofthemostpotentinflammatorychemoattractantsandinteractswithtwoC51receptors,C5aRandC5L2.MostoftheC5aeffectsaremediatedthroughC5aR.C5aRisknowntobecrucialintheinitiationofacuteinflammatoryresponses.C5areceptorisaseventransmembraneGTP-binding-protein-coupledreceptorthatbelongstotherhodopsinsupergenefamily.The45kDaC5areceptorisexpressedbyleukocytessuchasneutrophils,eosinophils,basophils,monocytes,dendriticcellsandmastcells.C5aRcanbeexpressedonbothimmuneandnonimmunecells.NonimmunecellsexpressingC5aRincludevascularendothelialcells,cardiomyocytesandbronchialepithelialcells.SignalingofC5aRinvolvesintracellularcalciummobilizationandactivationofamongothersproteinkinase-B(PKB)andproteinphospholipaseD(PLD)pathways.HighC5aRexpressionlevelshavebeenassociatedwiththepathogenesisofmanyinflammatoryconditions,autoimmuneandneurodegenerativediseaseslikerheumatoidarthritis,multiplesclerosis,atherosclerosisandinflammatoryboweldiseases.InvivoblockadeofC5aRgreatlyreducesinflammatoryinjury.Themonoclonalantibody20/70hasbeenusedinfunctionalstudiesandiscapabletopreventthebindingofC5atoitsreceptor.
Specifications:
| Catalognumber | HM1076 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies,Immunofluorescence |
| ApplicationNotes | FC:2x10^5cellswereblockedwithhumanandmurineIgGonicefor20’.Cellswerestainedwith10μgantibody20/70for45’inPBS/1.5%FCS.ForanalysiswithFACScellswerewashedwithPBS/1%formaldehyde(Ref.2). FS:BeforemigrationtowardsC5awasinduced,2x105cellswereincubatedfor30’with10μgantibody20/70inatranswellmigrationassay(Ref.2). IF:Cellswereculturedoncoverslips,fixedwith4%PFAfor20’,blockedwith3%BSAfor2handstainedwithantibody20/70for2h(Ref.6). |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | RBL-2H3transfectantsexpressingmurineC5aRonthecellsurface. |
| Isotype | RatIgG2b |
| Species | Mouse |
| Alias | C5aanaphylatoxinchemotacticreceptor1,C5aanaphylatoxinchemotacticreceptor,C5a-R Genename:C5ar1 |
| PositiveControl | Mouseneutrophils |
| NegativeControl | HEK293cells |
| References | 1.Shushakova,Netal;C5aanaphylatoxinisamajorregulatorofactivatingversusinhibitoryFcγRsinimmunecomplex-inducedlungdisease.JClinInvest2002,110:1823 2.Soruri,Aetal;CharacterizationofC5aRexpressiononmurinemyeloidandlymphoidcellsbytheuseofanovelmonoclonalantibody.ImmunolLett2003,88:47 3.Godau,Jetal;C5ainitiatestheinflammatorycascadeinimmunecomplexperitonitis.JImmunol2004,173:3437 4.Chen,NJetal;C5L2iscriticalfortheBIOLOGicalactivitiesoftheanaphylatoxinsC5aandC3a.Nature2007,446:203 5.Rittirsch,Detal;FunctionalrolesforC5areceptorsinsepsis.NatMed2008,14:551 6.Gueler,Fetal;Complement5aReceptorinhibitionimprovesrenalallograftsurvival.JAmSocNephrol2008,19:2302 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases,Nephrology |
