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商品描述
Description:Siglec-H,Mouse,mAb440c
Themonoclonalantibody440creactswithSiglec-H,acell-surfacereceptormoleculeselectivelyexpressedonmurinenaturalinterferonproducingcells(IPC),alsocalledmouseplasmacytoiddendriticcells(pDC).SiglecHisuniqueamongSialicacid-bindingIg-likelectins(Siglecs)proteinsbecauseitassociateswiththeadaptorproteinDAP12.DAP12recognizecertainvirusesandCpG-DNAthroughTLR9,resultinginsecretionofIFN-alpha,IL-12andproinflammatorychemokines.TogetherthesecytokinesandchemokinesrecruiteandactivateNKcellsandTcellsaswellasmodulatingtheantigenpresentingfunctionofdendriticcells(DC).IPCthemselvesalsofunctionasantigenpresentingcellsthatexpandmemoryTcellsandinduceTh1differentiation.ThereforeIPCmayprovideafirstlineofhostdefenseagainstviralinfectionsbyactivatingbothinnateandadaptiveresponsesinvivo.Themonoclonalantibody440cexclusivelyrecognizesmouseSiglec-Hinalllymphoidorgansunderbothnormalandinflammatoryconditions.Siglec-HisnormalpresentintheTcellzoneoflymphnodesandspleen.IncubationofIPCwithantibody440cinvitrooradmiNISTrationofantibody440cinvivoreducessecretionofIFN-alphainresponsetoCpGDNAwithoutcausingIPCdepletion.
Specifications:
Catalognumber | HM1075 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
Application | Flowcytometry,Frozensections,Functionalstudies,Paraffinsections |
Use | Forimmunohistologyandflowcytometrydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ForinhibitionofIFN-alphasecretiondilutionshavetobemadeaccordingtotheamountsofSiglec-Htobeinactivated. |
Isotype | RatIgG2b |
Species | Mouse |
References | 1.Blasius,Aetal;Siglec-HisanIPC-specificreceptorthatmodulatestypeIIFNsecretionthroughDAP12.Blood2006,107:2474 2.Blasius,Aetal;Acell-surfacemoleculeselectivelyexpressedonmurinenaturalinterferon-producingcellsthatblockssecretionofinterferon-alpha.Blood2004,103:4201 3.Colonna,Metal;Plasmacytoiddendriticcellsinimmunity.NatImmunol2004,5:1219 4.Seth,Setal;CCR7EssentiallyContributestotheHomingofPlasmacytoidDendriticCellstoLymphNodesunderSteady-StateAsWellAsInflammatoryConditions.JImmunol2011,186:3364 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Infectiousdiseases |