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蚂蚁淘在线 / 品牌中心 / Hycult Biotech / Hycult Biotech/Macrophages F4/80, Mouse, mAb BM8, FITC/HM1066F/100 µg
商品描述
Description:MacrophagesF4/80,Mouse,mAbBM8,FITC
ThemonoclonalantibodyBM8recognisesa125kDextracellularmacrophagemembranemolecule,highlyrestrictedtomaturemacrophagesubpopulationsresidingintissue.ThismurineF4/80glycoproteincontainsseven-transmembrane(TM7)regions,whichanchortheproteininthecellmembrane,andtherebyshowssimilarityinthisregiontoG-protein-coupledreceptors.TheF4/80moleculesharesoverallstructuralhomologytoothermembersoftheepidermalgrowthfactor(EGF)-TM7family.Theantigenisdetectedontissuefixedmacrophagesinallorganstestedsofar(spleen,lymphnodes,thymus,liver,skin).ItisalsopresentonLangerhanscellsintheskinandKupffercellsintheliver.Itisabsentongranulocytes,lymphocytesandtrombocytes.TheexpressionofF4/80increasesuponmaturationofmacrophageprecursorsinbonemarrowandbloodaswellasinontogeny.
ThemonoclonalantibodyBM8istheonlymacrophageMarkerthatisabletodistinguishnon-destructivefromdestructiveinflammationprocessesinthepancreas.FurThermoreitisauniquehistologicalmarkeroftheprogressionfromperi-insulitistobeta-celldestructionanddiabetesinamousediabetesmodel.ThemonoclonalantibodyBM8crossreactswithhumanheartmacrophages.
ThemonoclonalantibodyBM8istheonlymacrophageMarkerthatisabletodistinguishnon-destructivefromdestructiveinflammationprocessesinthepancreas.FurThermoreitisauniquehistologicalmarkeroftheprogressionfromperi-insulitistobeta-celldestructionanddiabetesinamousediabetesmodel.ThemonoclonalantibodyBM8crossreactswithhumanheartmacrophages.
Specifications:
Catalognumber | HM1066F |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedFITCconjugatedantibodysolutioninPBS,containing1.0%bovineserumalbuminand0.02%sodiumazide. |
Conjugate | FITC |
Application | Flowcytometry,Frozensections,Paraffinsections,Westernblot |
ApplicationNotes | W:Mousebone-marrowderivedmacrophages;non-reduced;~125kDa(Ref1);reductionwith2-mercaptoethanoldestroysBM8antigen. F:tissueembeddedinOCTTissueTec;fixedwithacetonefor10minatRT;incubationwith0.02MsodiumazideinPBScontaining0.1%H2O2for10minatRTtodestroyendogenousperoxidase;spleenaspositivecontrol.P:fixationin10%neutralbufferedformalinfor24h;blockingwithnon-immunizedgoatserum;microwavedfor6minincitratebuffer;splenicmacrophagesaspositivecontrol(Ref3). FC:fixedwith1%paraformaldehyde(Ref1). |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Immunogen | BALB/cmacrophagesobtainedfrom14-day-oldbonemarrowcellcultures |
Isotype | RatIgG2a |
Species | Mouse |
PositiveControl | Mousemacrophages |
NegativeControl | Mousefibroblastsorgranulocytes |
References | 1.Malorny,Uetal;Amonoclonalantibodyagainstanantigenpresentonmousemacrophagesandabsentfrommonocytes.CellTissueRes1986,243:421 2.Leenen,Petal;Markersofmousemacrophagedevelopmentdetectedbymonoclonalantibodies.JImmunolMethods1994,174:5 3.Mackler,Aetal;Macrophagetraffickingintheuterusandcervixprecedesparturitioninthemouse.BiolReprod1999,61:879 4.Schaller,Eetal;InactivationoftheF4/80glycoproteininthemousegermline.MolCellBiol2002,22:8035 |
StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Cardiologyandmetabolism,Infectiousdiseases |