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商品描述
Description:C3,Mouse,mAb11H9
Themonoclonalantibody11H9recognizesmousecomplementcomponentC3anditsactivationproducts,C3b,iC3b,C3dandC3dg.Thecomplementsystemisanimportantpartofthehumoralresponseininnateimmunity,consistingofthreedifferentpathways.Thethirdcomplementcomponent,C3,iscentraltotheclassical,alternativeandlectinpathwaysofcomplementactivation.Activationproductsofthecomplementcascadecontainneo-epitopesthatarenotpresentintheindividualnativecomponents.ThecomplementfactorC3consistsofanalpha-andabeta-chain.
ThesynthesisofC3istissue-specificandismodulatedinresponsetoavarietyofstimulatoryagents.C3isthemostabundantproteinofthecomplementsystemwithserumproteinlevelsofabout1.3mg/ml.AninheriteddeficiencyofC3predisposesthepersontofrequentassaultsofbacterialinfections.Inulcerativecolitis,andidiopathicchronicinflammatoryboweldisease,thedepositionofC3inthediseasedmucosahasbeenreported.
ProteolysisbycertainenzymesresultsinthecleavageofC3intoC3aandC3b.C3bbecomesattachedtoimmunecomplexesandisfurthercleavedintoiC3b,C3c,C3dgandC3f.Themonoclonalantibody11H9recognizesbothintactC3anditscleavedproductsC3b,iC3b,C3dandC3dg.Theseactivationproductsarepresentinacuteaswellaschronicinflammatoryconditions.Inchronicinflammatorycondition,primarilytheC3dgproductresidesattheplaceofinflammation(C3cbeingcleared)whichisrecognizedbyantibody11H9.Thechronicprocessing/activationofC3istakingplaceatalowerlevel,whichwouldreducedetectionoftheC3fragmentsC3b,iC3b,andC3c.
ThesynthesisofC3istissue-specificandismodulatedinresponsetoavarietyofstimulatoryagents.C3isthemostabundantproteinofthecomplementsystemwithserumproteinlevelsofabout1.3mg/ml.AninheriteddeficiencyofC3predisposesthepersontofrequentassaultsofbacterialinfections.Inulcerativecolitis,andidiopathicchronicinflammatoryboweldisease,thedepositionofC3inthediseasedmucosahasbeenreported.
ProteolysisbycertainenzymesresultsinthecleavageofC3intoC3aandC3b.C3bbecomesattachedtoimmunecomplexesandisfurthercleavedintoiC3b,C3c,C3dgandC3f.Themonoclonalantibody11H9recognizesbothintactC3anditscleavedproductsC3b,iC3b,C3dandC3dg.Theseactivationproductsarepresentinacuteaswellaschronicinflammatoryconditions.Inchronicinflammatorycondition,primarilytheC3dgproductresidesattheplaceofinflammation(C3cbeingcleared)whichisrecognizedbyantibody11H9.Thechronicprocessing/activationofC3istakingplaceatalowerlevel,whichwouldreducedetectionoftheC3fragmentsC3b,iC3b,andC3c.
Specifications:
| Catalognumber | HM1045 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide. |
| Application | Flowcytometry,Frozensections,Immunoassays,Immunofluorescence,Westernblot |
| ApplicationNotes | IA:Antibody11H9wasusedascoating0.5µg/wellinPBS. W:A-reducedsampletreatmentandSDS-PAGEwasused.Thebandsizesare~110-115kDa(;-chain),~80and70kDa(Ref.1). F:-Tissuesectionswerefixedinacetone.Antigenretrievalwasperformedusing50%formicacidfor5minormicrowaved(700W)inantigenunmaskingsolution(Ref1,3). IF:Fixationin4%paraformaldehydeinPBSpH7.4.Vibratomesectionsof4 µm;m.Pretreatedwith3%hydrogenperoxidefor20mintoquenchendogenousperoxidases.Microwavedinantigenunmaskingsolutionfor2-5minutesasantigenretrieval.(Ref.3). |
| Use | ForWesternblotting,flowcytometryandimmunohistologydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | C57BL/6thymocytessaturatedwithratanti-Thy-1monoclonalantibodyofIgG2bsubclass(RmT1) |
| Isotype | RatIgG2a |
| Species | Mouse |
| Alias | omplementcomponentC3,HSE-MSF Genename:C3 |
| PositiveControl | MPL-lprkidneycellsF:-AspositivecontrolbrainsectionsofAPPQmicewereusedandasnegativecontrolisotypematchedmaterial |
| References | 1.Kremmer,Eetal;Monoclonalantibodiestocomplementcomponentswithouttheneedoftheirpriorpurification.II.AntibodiestomouseC3andC4.Hybridoma1990,9:309 2.Pan,Wetal;CR3(CD11b/CD18)isthemajormacrophagereceptorforIgMantibodymediatedphagocytosisofAfricantrypanosomes:Diverseeffectonsubsequentsynthesisoftumornecrosisfactoraandnitricoxide.MicrobInfect2006,8:12093.Zhou,Jetal;ComplementC3andC4expressioninC1qsufficientanddeficientmousemodelsofAlzheimer"sdisease.JNeuRochem2008,106:2080 4.Heurich,Betal;ComplementupregulationandactivationonmotorneuronsandneuromuscularjunctionintheSOD1G93Amousemodeloffamilialamyotrophiclateralsclerosis.JournalofNeuroimmunology2011,235:1045.Lewis,Retal;CD55DeficiencyProtectsagainstAtherosclerosisinApoE-DeficientMiceviaC3aModulationofLipidMetabolism.AmJPath2011,179:1601 6.Lesher,Aetal;CombinationofFactorHMutationandProperdinDeficiencyCausesSevereC3Glomerulonephritis.AmSocNephrol2013,24:537.Antunes,Aetal;ThePhosphocarrierProteinHPrContributestoMeningococcalSurvivalduringInfection.PLosOne2015,11:e0162434 8.Ramaglia,Vetal;Complementactivationandexpressionduringchronicrelapsing.ClinExpImmunol2015,180:432 |
| StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Infectiousdiseases,Nephrology |
