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商品描述
Description:MASP-3,Human,ELISAkit
Themannan-bindinglectin(MBL)pathwayofthecomplementsystemisinitiatedbythebindingofMBLorficolintospecificcarbohydratestructuresfoundonthesurfaceofavarietyofmicroorganisms.ThisbindingisfollowedbytheactivationofMBL-associatedserineproteases(MASPs).TherearefourknownMASPs:MASP-1,MASP-2,MASP-3andMap19(alsoknownassMAP,asmall,separatelysynthesizedfragmentofMASP-2).MASPsaresecretedassinglechainprecursorsandareprocessedintoaheavychain(A)andcatalyticlightchain(B)linkedbydisulfidebonds.AllMASPsformhomo-dimersandindividuallyassociatewithMBLandtheficolinsinaCa2+-dependentmanner.MASP-3isanalternativelysplicedproductfromtheMASP-1gene,containinganidenticalAchain,butanentirelydifferentBchain.MASP-3isanapproximately100kDaproteinandassociateswithbothMBLandL-ficolininthepresenceofCa2+.TheMASP3dimercanbedisruptedbyEDTA.MASP-3isexpressedmainlybutnotexclusivelyintheliver.LiverexpressedMASP-3issecretedintotheblood.UnlikeMASP-2,noproteolyticactivityoncomplementproteinssC2,C3andC4forMASP-3hasbeendetected.MASP-3exertsinhibitoryactivityonMASP-2function.However,nonaturalsubstratesforMASP-3areknown.MASP-3concentrationinhealthyhumanserumisnormallydistributedwithameanvalueof3796ng/ml.MASP-3ispresentatbirthatapproximately80%ofthelevelmeasured6,9and12monthsafterbirth.TheMASP-3levelisstablethroughouta1-yearperiod.ThehighexpressionofMASP-3inastrocytesindicatesthatMASP-3mightalsobeinvolvedinotherfunctionsthatcouldbeimportantinthebrain.Inmultiplesclerosispatients,highMASP-3levelsseemtohaveapositiveeffect(medianMASP-3levels:4539ng/ml).MASP-3deficienciesarecurrentlynotreported.
Specifications:
| Catalognumber | HK339-01 |
|---|---|
| Producttype | Assays |
| Quantity | 1x96det. |
| Standardrange | 8–500ng/ml |
| Detectionlevel | 8ng/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Application | ThehumanMASP-3ELISAkitistobeusedfortheinvitroquantitativedeterminationofbothnaturalandrecombinanthumanMASP-3inserumandplasmasamples. |
| Disease | Autoimmunity,Infectiousdiseases |
| Principle | TheMASP-3ELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof4½hours.Theefficientformatof2plateswithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedMASP-3.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsoftheMASP-3standards(log).TheMASP-3concentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| Linearity | Thelinearityoftheassaywasdeterminedbyseriallydilutingasamplecontaining250ng/mlhumanMASP-3.Thedilutedsamplesweremeasuredintheassay.Thelineobtainedaslopeof0.970andacorrelationcoefficientof0.999. |
| Recovery | Normalhumanbloodsamples,containingbaselinelevelsofMASP-3,werespikedwithrecombinantMASP-3inconcentrationsof100and10ng/ml.SampleswithandwithoutMASP-3wereincubatedfor30minutesat37°C.SamplesweremeasuredusingtheELISA.ValuesforMASP-3rangedbetween93%and116%(mean110%). |
| StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| References | 1.Frauenknecht,Vetal;Plasmalevelsofmannan-bindinglectin(MBL)-associatedserineproteases(MASPs)andMBL-associatedproteinincardio-andcerebrovasculardiseases.Clinical&ExperimentalImmunology2013 |
