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商品描述
Description:TNF-alpha,Human,ELISAkit
Tumornecrosisfactoralpha(TNF-alpha),ahomotrimeric17KDprotein,isapotentmediatorofinflammatoryandmetabolicfunctions.TNF-alphawasoriginallydetectedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,TNF-alphahasbeenimpliedascentralmediatorinshockinducedbygramnegativemicro-organisms.ThecytokineTNFisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses:itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesbutitalsoinducesonitsturntheproductionofmanycytokines.SignaltransductionoccursviatwotypesofTNF-receptors,thep55(RI)andp75(RII)TNF-receptors.Thereceptorsdifferstronglyintheirintra-cellularsignalingpathways.TheTNF-alphatrimerinteractswitheitherofthetwotypesofTNF-RleADIngtoreceptorcross-linking.
Specifications:
| Catalognumber | HK307-01 |
|---|---|
| Producttype | Assays |
| Quantity | 1x96det. |
| Standardrange | 8-2,000pg/ml |
| Detectionlevel | 10pg/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Application | ThehumanTNF-αELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanTNF-αinserum,plasma,andcellculturesupernatantsamples. |
| Disease | Autoimmunity,Gastroenterology |
| Principle | ThehumanTNF-alphaELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatofaplatewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedhumanTNF-alpha.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofcitricacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanTNF-alphastandards(log).ThehumanTNF-alphaconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| References | 1.Molnar,Letal;DetectionofTNFalphaexpressioninthebonemarrowanddeterminationofTNFalphaproductionofperipheralbloodmononuclearcellsinmyelodysplasticsyndrome.PatholOncolRes2000,6:18 2.Younis,Aetal;Staphylococcusaureusleucocidin,avirulencefactorinbovinemastitis.JDairyResearch2005,72:188 |
