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蚂蚁淘在线 / 品牌中心 / Hycult Biotech / Hycult Biotech/Nitrotyrosine, mAb HM.11, immuno assay antibody/HM5001-IA/0.5 mg
商品描述
Description:Nitrotyrosine,mAbHM.11,immunoassayantibody
ThemonoclonalantibodyHM.11recognizesmodifiedaminoacidnitrotyrosineinalldifferentspecies.NitrotyrosineisformedintissuesinpresenceoftheactivemetaboliteNOandisastableendproductofnitrosylationoftyrosine.Inflammationischaracterizedbyincreasednitricoxide(NO)production.NOreactsrapidlywithsuperoxidetoformperoxynitrite.AtphysiologicalpHandinthepresenceoftransitionmetals,peroxynitriteundergoesheterolyticcleavagetoformhydroxylanionandnitroniumion,thelatterofwhichnitratesproteintyrosineresidues.Thepresenceofnitrotyrosinehasbeendetectedinvariousinflammatoryprocessesincludingatheroscleroticplaques,AmyotrophicLateralSclerosis(ALS)andMultipleSclerosis(MS).Thus,thepresenceofnitrotyrosineonproteinscanbeusedasaMarkerforperoxynitriteformationinvivoandconsequentlyasamarkerofNO-mediatedtissuedamage.ThemonoclonalantibodyHM.11recognizesnitrotyrosine,bothwiththefreeaminoacidaswellaswithproteinscontainingnitrotyrosine
Specifications:
| Catalognumber | HM5001-IA |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel). |
| Application | Frozensections,Immunoassays,Paraffinsections,Westernblot |
| ApplicationNotes | F:alsocytospins:acetonefixation10min-20 °C;blockendogenousperoxidaseby0.3%H2O2inPBS(ormethanolforintracellularstaining);blockingwith10%NGSor5%BSAfor30min.useatassaydependentconcentration P:10%formalinfixation;3%H2O2toblockendogenousperoxidases;CitratebufferpH6.0for1minat100 °Casantigenretrievaltreatment;useatassaydependentconcentration(1:200/400)-(Ref1) W:reducedandno-reducedsamples;blockwith5%BSAorskimmedmilk,useatassaydependentconcentration |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | NitratedKLH |
| Isotype | MouseIgG2b |
| Species | N/A |
| PositiveControl | W:mousekidneylysate,mouseopticnerve,retina,spinalcordandbrainlysates,rataortalysateP:humanlungtissue |
| References | 1.TerSteege,Jetal;NitrotyrosineinplasmaofceliacdiseasepatientsasdetectedbyanewsandwichELISA.FreeRADIcBiolMed1998,25:9532.Klausz,Getal;LocalandperipheralcytokineresponseandCagAstatusofHelicobacterpylori-positivepatientswithduodenalulcer.EurCytokineNetw2003,14:143 3.Casoni,Fetal;Proteinnitrationinamousemodeloffamilialamyotrophiclateralsclerosis.JBiolChem2005,280:162954.Han,Fetal;Proteinnitrationandpoly-ADP-ribosylationinbrainafterrapidexsanguinationscardiacarrestinaratmodelofemergencypreservationandresuscitation.Resuscitation2008,79:301 5.Tsuhako,Hetal;Tempolamelioratesmurineviralencephalomyelitisbypreservingtheblood-brainbarrier,reducingviralload,andlesseninginflammation.FreeradicBiolMed2010,48:7046.Brunelli,Letal.Exploratoryinvestigationonnitro-andphospho-proteomecerebellumchangesinhyperammonemiaandhepaticencephalopathyratmodels.Metabolicbraindisease2012,27:37 7.Nardo,Getal.AmyotrophicLateralSclerosisMultiproteinBiomarkersinPeripheralBloodMononuclearCells.Plosone2011,6:e255458.Yip,P.K.etal.TheOmega-3FattyAcidEicosapentaenoicAcidAcceleratesDiseaseProgressioninaModelofAmyotrophicLateralSclerosis.PlosOne2013,8:61626 |
| StorageandstABIlity | Productshouldbestoredat-20 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| Disease | Autoimmunity,Cardiologyandmetabolism |
