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蚂蚁淘在线 / 品牌中心 / GenWay / GenWay/Total Bile Acids Assay (Dual vial liquid stable and powder) (NBT method)/GWB-BQK090/600 Tests
商品描述
Description:
Description | TheTotalBileAcidsColorimetricAssaykitisforthedeterminationofserumtotalbileacids(TBA).TotalbileacidsaremetabolizedintheliverandhenceserveasaMarkerfornormalliverfunction.Serumtotalbileacidsareincreasedinpatientswithacutehepatitis,chronichepatitis,liversclerosisandlivercancer.InthepresenceofNAD,theenzyme3-αhydroxysteroiddehydrogenase(3-αHSD)convertsbileacidsto3-ketosteroidsandNADH.TheNADHformedreactswithnitrotetrazoliumblue(NBT)toformaformazandyeinthepresenceofdiaphoraseenzyme.Thedyeformationismonitoredbymeasuringabsorbanceat540nmandisdirectlyproportionaltothebileacidsconcentrationintheserumsample. |
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Species | Human |
SampleType | SerumorPlasma |
AdditionalInformation:
Name | TotalBileAcidsAssay(Dualvialliquidstableandpowder)(NBTmethod) |
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ShortDescription | TheTotalBileAcidsColorimetricAssaykitisforthedeterminationofserumtotalbileacids(TBA).Totalbileacidsaremetabolizedintheliverandhenceserveasamarkerfornormalliverfunction.Serumtotalbileacidsareincreasedinpatientswithacutehepatitis,chronichepatitis,liversclerosisandlivercancer.InthepresenceofNAD,theenzyme3-αhydroxysteroiddehydrogenase(3-αHSD)convertsbileacidsto3-ketosteroidsandNADH.TheNADHformedreactswithnitrotetrazoliumblue(NBT)toformaformazandyeinthepresenceofdiaphoraseenzyme.Thedyeformationismonitoredbymeasuringabsorbanceat540nmandisdirectlyproportionaltothebileacidsconcentrationintheserumsample. |
Reactivity | Human |