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蚂蚁淘在线 / 品牌中心 / GenWay / GenWay/Total Bile Acids Assay (Dual vial liquid stable) (Enzyme cycling method)/GWB-BQK086/430 Tests
商品描述
Description:
Description | TheTotalBileAcidsEnzymaticAssayKitisintendedfortheinvitroquantitativedeterminationofserumtotalbileacids(TBA).Totalbileacidsaremetabolizedintheliverandhence,serveasaMarkerfornormalliverfunction.Serumtotalbileacidsareincreasedinpatientswithacutehepatitis,chronichepatitis,liversclerosisandlivercancer.Thereagentsoftheassaykitarestableliquidformulationthatallowseaseofusecoupledwithenhancedperformancecharacteristics.InthepresenceofThio-NAD,theenzyme3-α.hydroxysteroiddehydrogenase(3-α-HSD)convertsbileacidsto3-ketosteroidsandThio-NADH.ThereactionisreversIBLeand3-α-HSDcanconvert3-ketosteroidsandThio-NADHtobileacidsandThio-NAD.InthepresenceofexcessNADH,theenzymecyclingoccursefficientlyandtherateofformationofThio-NADHisdeterminedbymeasuringspecificchangeofabsorbanceat405nm. |
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Species | Human |
SampleType | SerumorPlasma |
AdditionalInformation:
Name | TotalBileAcidsAssay(Dualvialliquidstable)(Enzymecyclingmethod) |
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ShortDescription | TheTotalBileAcidsEnzymaticAssayKitisintendedfortheinvitroquantitativedeterminationofserumtotalbileacids(TBA).Totalbileacidsaremetabolizedintheliverandhence,serveasamarkerfornormalliverfunction.Serumtotalbileacidsareincreasedinpatientswithacutehepatitis,chronichepatitis,liversclerosisandlivercancer.Thereagentsoftheassaykitarestableliquidformulationthatallowseaseofusecoupledwithenhancedperformancecharacteristics.InthepresenceofThio-NAD,theenzyme3-α.hydroxysteroiddehydrogenase(3-α-HSD)convertsbileacidsto3-ketosteroidsandThio-NADH.Thereactionisreversibleand3-α-HSDcanconvert3-ketosteroidsandThio-NADHtobileacidsandThio-NAD.InthepresenceofexcessNADH,theenzymecyclingoccursefficientlyandtherateofformationofThio-NADHisdeterminedbymeasuringspecificchangeofabsorbanceat405nm. |
Reactivity | Human |