Description:

Description:RecombinantHEK293celllineexpressingratPDE7A(phosphodiesterase7A,accessionnumberNM_031080).Format:Eachvialcontains1X10(6)cellsin1mlof10%DMSO.Functionalvalidation:N-terminalFLAG-taggedratPDE7AhasbeenstablyexpressedinHEK293celllineanditsexpressionwasconfirmedbyWesternblotting.TheregulationofintracellularlevelofcAMPbyratPDE7AinratPDE7Astably-expressedHEK293cellswascharacterizedbyacell-basedreporterassayusingpCRE-lucreportervector.pCRE-luccontainsaluciferasegenethatisunderthecontrolofthecAMPresponseelement(CRE).WhencellstransientlytransfectedwithpCRE-lucreporterwereactivatedbyforskolin,thelevelofcAMPwasupregulatedinparentalHEK293cellsinducingtheexpressionofluciferasereporterwhereasratPDE7A-HEK293cellsshowedreductioninthelevelofcAMP,resultinginloweredlevelsofluciferaseexpression.InhibitionofPDE7AactivitybyBRL50481,aPDE7Ainhibitor,restoredthecAMPlevel,resultinginhigherluciferaseactivity.Storage:Storecellsinliquidnitrogenuponarrival.Avoidfreeze/thawcycles.References:Malik,R.etal.(2008)Appl.Microbiol.Biotechnol.77(5):1167-1173.FanChung,K.(2006).Eur.J.Pharmacol.533(1-3):110-117.

Host:HEK293(human)

GINum:NM_031080

Format:aqueoussolutioncontainingDMSO

ScientificCategory:PDEcellline

Species:rat

AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

Name	PDE7A-HEK293RatRecombinantCellLine
RelatedProductNames	PDE,phosphodiesterase
Source	HEK293(human)
GINumber	NM_031080
Format	AqueousSolutionContainingDmso
Reactivity	rat
Storage	liquidN2
IntendedUse	ResearchUseOnly