Description:

Description:RecombinantHEK293celllineexpressinghumanPDE7A(phosphodiesterase7A,accessionnumberNM_002603).Format:Eachvialcontains1X10(6)cellsin1mlof10%DMSO.Functionalvalidation:N-terminalFLAG-taggedhumanPDE7Ahasbeenstablyexpressedinahumanembryonickidney(HEK293)cellline.PDE7AexpressionwasconfirmedbyWesternblotting.TheregulationofintracellularlevelofcAMPbyPDE7AinPDE7Astably-expressedHEK293cellswascharacterizedbyacell-basedreporterassayusingpCRE-lucreportervector.WhencellstransientlytransfectedwithpCRE-lucreporterwereactivatedbyforskolin,thelevelofcAMPwasupregulatedinparentalHEK293cellsinducingtheexpressionoftheluciferasereporterwhereashPDE7A-HEK293cellsshowedreductioninthelevelofcAMPthatresultedinloweredexpressionofluciferase.InhibitionofPDE7AactivitybyBRL50481,aPDE7Ainhibitor,restoredthecAMPlevel,resultinginhigherluciferaseactivity.Storage:Storecellsinliquidnitrogenuponarrival.Avoidfreeze/thawcycles.References:Malik,R.etal.(2008)Appl.Microbiol.Biotechnol.77(5):1167-1173.FanChung,K.(2006).Eur.J.Pharmacol.533(1-3):110-117.

Host:HEK293(human)

GINum:NM_002603

Format:aqueoussolutioncontainingDMSO

ScientificCategory:PDEcellline

Species:human

AdditionalInformation:

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Name	PDE7A-HEK293HumanRecombinantCellLine
RelatedProductNames	PDE,phosphodiesterase
Source	HEK293(human)
GINumber	NM_002603
Format	AqueousSolutionContainingDmso
Reactivity	Human
Storage	liquidN2
IntendedUse	ResearchUseOnly