Description:

Description:Mouse,recombinant,N-terminalHis-GST-tagDescription:MouseCotkinase(MAP3K8)GenBankAccessionNo.NM_007746,a.a.30-397,withN-terminalHis-GST-tag,expressedinaBaculovirusinfectedSf9cellexpressionsystem.ThetheoreticalMWis70kDa.SpecificActivity:8.5pmole/min/mgAssayConditions:Reactionwasdoneinakinasebuffer(40mMTris,10mMMgCl2,0.1mg/mlBSA)containing5?ginactiveMEK1,20?MATP,andmCOTfor60minat30°C.TheamountofATPtransferredwasmeasuredbyKinase-Gloplus(Promega).Formulatedin:40mMTris-HCl,pH8.0,110mMNaCl,2.4mMKCl,5mMglutathione,134mMimidazole,3mMDTTand20%glycerol.Stability:>6monthsat-80°CReferences:1.Jeong,J.H.etal.,PLoSOne.2011Jan18;6(1):e16205.2.Soria-Castro,I.et.al.JBiolChem.2010Oct29;285(44):33805-15.

Host:baculovirus

Tag:N-terminalHis-GSTtag

GINum:NM_007746

MW:70kDa(calculated)

Format:aqueousbuffersolution

Formula:40mMTris-HCl,pH8.0,110mMNaCl,2.4mMKCl,5mMglutathione,134mMimidazole,3mMDTTand20%glycerol.

ScientificCategory:Kinase/Serine-Threonine

Species:mouse

AdditionalInformation:

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Name	Cot/Tpl2,mouseRecombinantProtein
RelatedProductNames	Cot/Tpl2,mouseRecombinantProtein
MolecularWeight	70kDa(calculated)
Purity	40mMTris-HCl,pH8.0,110mMNaCl,2.4mMKCl,5mMglutathione,134mMimidazole,3mMDTTand20%glycerol.
Source	baculovirus
GINumber	NM_007746
Format	AqueousBufferSolution
Reactivity	Mouse
Storage	-80°C
IntendedUse	ResearchUseOnly