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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Description:
Description:TheFluorogenicSIRT1AssayKitisacompleteassaysystemdesignedtomeasureSirtuin1(SIRT1)activityforscreeningandprofilingapplications.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100uluorescentSIRT1(Sir2)activitymeasurements.Inaddition,thekitincludespurifiedSIRT1enzymeandaSIRTinhibitor,Nicotinamide,foruseasapositiveandnegativecontrol,respectively.TheFluorogenicSIRT1AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheSIRT1activitylevel.First,theSIRTfluorometricsubstrate(SIRTsubstrate1),containinganacetylatedlysinesidechain,isincubatedwithasamplecontainingpurifiedSIRT1enzyme.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.Components:SIRT1humanrecombinantenzyme,50?g;FluorogenicSIRTsubstrate1,50ul@5mM;NicotinamideAdenineDinucleotide(NAD+),50ul@50mM;Nicotinamide,500ul@10mM;2xSIRTDeveloper(contains2mMNicotinamide),6ml;SIRTassaybuffer,10ul;1black,lowbindingNUNCblackmicrotiterplate.APPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.REFERENCES:1.A.Itoetal.(2001)EMBOJ.201331.2.N.A.Barlevetal.(2001)Mol.Cell81243.3.A.Itoetal.(2002)EMBOJ.216236.Format:assaykit
ScientificCategory:DeacetylaseAssay
AdditionalInformation:
Name | Sirt1(Sir2)AssayKit |
---|---|
RelatedProductNames | sirt1,Sir2,Sir2a |
Format | AssayKit |
Storage | Mixedstorage |
IntendedUse | ResearchUseOnly |