Description:

Description:TheFluorogenicProlylOligoPeptidase(POP)AssayKitisacompleteassaysystemdesignedtomeasureactivityofthepurifiedPOPenzyme.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100reactions.TheFluorescentPOPActivityAssayeliminatesthedealingwithrADIoactivematerialsandchromatographyintraditionalassays.PurifiedhumanrecombinantPOPisincludedinthekitasapositivecontrol.Usingthiskit,onlyonesimplestep,inwhichthefluorometricsubstrateisincubatedwithpurifiedPOP,isneededtoanalyzethePOPactivitylevel.Theresultingfluorescentproductcanthenbemeasuredwithamicrotiter-platefluorimeter.COMPONENTS:POPassaybuffer:10ulFluorogenicPOPsubstrate:100µl@0.5mMRecombinanthumanPOP:Xµgfor100reactionsFluorogenicstandard500µl@50µMOneblackplate(VWRcatalogno.62408-936).APPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.

Format:assaykit

ScientificCategory:Protease/Substrate

AdditionalInformation:

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Name	POPassaykit
RelatedProductNames	POPassaykit
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly