

Description:
baculoFECTIN isanewnon-toxictransfectionreagentthathasbeenoptimizedforuseininsectcellstoproducegreaterlevelsoftransfection–leADIngtohighlevelproteinexpression.
AlthoughoptimisedforinsectcelllinessuchusSf9,Sf21andT.ni,thereagentwillalsoworkwithothercelllinesandprimarycellstoo,includingvariousmammaliancelllines. baculoFECTIN consistsoftwocomponents,apositivelychargedpolymerwithDNA-bindingcapacitythatisembeddedintothesecondcomponent,anovelporousnanoparticle.ThecompletenanoparticlecomplexprotectstheboundDNAfromdegradationbynucleases.OnlytheDNAnanoparticlecomplexwithitsuniquesizecanbepreferentiallytakenupbythecells.Withinthecells,thereleasedDNA-nanoparticlecomplexesareprotectedagainstDNAdegradation.
Themainbenefitsof baculoFECTIN comparedwithconventionallipid-basedproductsarethatithasaquick,effectiveandeasyprotocol,fromco-transfectiontohightitreseedstock;itisnon-toxicsocellhealthandviABIlityismaintained;andthenanoparticle/polymerduplexoptimizesentryintocellsandstabilizestheDNAwithintransfectedinsectcells.Stableforuptoayear, baculoFECTIN istheperfectcompaniontoourleadingbaculovirusexpressionsystem,flashBAC .Itisidealforhigh-throughputtransfectionexperiments.
AdditionalInformation:
Name | baculoFECTINIITransfectionReagent150ul |
---|---|
Concentration | LotSpecific |
Stability | 6months |
Storage | 4°C |
IntendedUse | ResearchUseOnly |