Description:

Description:alpha-Ketoglutarate(alpha-KG)isakeyintermediateintheKrebscycle,comingafterisocitrateandbeforesuccinylCoA.Anapleroticreactionsreplenishthecyclebysynthesizingalpha-KGfromtransaminationofglutamate,orthroughtheactionofglutamatedehydrogenase.alpha-KGisanimportantnitrogentransporter.Beingakeyintermediate,itisoneoftheorganicacidsmeasuredinnewbornsasanindicatorofinbornerrorsofmetabolism.GenWay"salpha-KetoglutarateAssayKitprovidesasimple,sensitiveandrapidmeansforquantifyingalpha-KGinavarietyofsamples.Intheassay,alpha-ketoglutarateistransaminatedwiththegenerationofpyruvatewhichisutilizedtoconvertanearlycolorlessprobetobothcolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Thealpha-KetoglutarateAssayKitisusefulfordetectingalpha-ketoglutarateintherangeof0.01to10nmoles.
Summary:
-Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)
-Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids
-Speciesreactivity-Mammalian
-Applications-Thealpha-KetoglutarateAssayKitisusefulfordetectingalpha-ketoglutarateintherangeof0.01to10nmoles.
Kitcomponents:
-alpha-KGAssayBuffer
-alpha-KGOxiRedProbe(inDMSO)
-alpha-KGConvertingEnzyme
-alpha-KGDevelopmentEnzymeMix
-alpha-KGStandard(10µmol)
FeaturesandBenefits:
-Simpleprocedure;takes~30minutestocompletetheassay
-Fastandconvenient
-Highlysensitiveassaysformeasuringalpha-Ketaglutaratelevelsinbiologicalsamples
ShippingConditions:Gelpack

AdditionalInformation:

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Name	Alpha-KetoglutarateAssayKit
RelatedProductNames	Ketoglutarate-AlphaAssayKit,Ketoglutarate,-Alphaassay,Ketoglutarate,-Alphadetectionkit,Ketoglutarate-Alphadetection
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly