Description:

Description:FattyAcidsplayveryimportantrolesinnormalmetabolismandmanydiseasedevelopments.Theyareprecursorstoanumberofbioactiveclassesofcompoundssuchasprostaglandins,leucotrienesandothers,andhavebeenimplicatedindiversefunctionssuchasautism,immunesystemandinflammationresponse.GenWay"sFreeFattyAcidQuantificationKitprovidesaconvenient,sensitiveenzyme-basedmethodfordetectingthelong-chainfreefattyacidsinvariousBIOLOGicalsamples,suchasserum,plasmaandotherbodyfluids,food,growthmedia,etc.Intheassay,FattyAcidsareconvertedtotheirCoAderivatives,whicharesubsequentlyoxidizedwiththeconcomitantgenerationofcolororfluorescence.C-8(octanoate)andlongerfattyacidscanthenbeeasilyquantifiedbyeithercolorimetric(spectrophotometryatλ=570nm)orfluorometric(atEx/Em=535/587nm)methodswithdetectionlimit2µMfreefattyacidinvarietysamples.
Summary:
-Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)
-Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherbiologicalfluids
-Speciesreactivity-Mammalian
-Application-Thekithasadetectionlimit2µMfreefattyacidinvarietysamples.
Kitcomponents:
-FattyAcidAssayBuffer
-FattyAcidProbe(lyophilized)
-Dimethylsulfoxide(DMSO;Anhydrous)
-ACSReagent
-EnzymeMix
-Enhancer
-PalmiticAcidStandard(1nmol/µl)
FeaturesandBenefits:
-Simpleprocedure;takes~40minutes
-Fastandconvenient
-Kitcontainsallnecessaryreagentsforrapid,sensitiveandaccuratemeasurementofADIpolysisincellculturesamples
ShippingConditions:Gelpack

AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

Name	FreeFattyAcidQuantificationKit
RelatedProductNames	FattyAcidAssayKit,Fattyacidassay,fattyacid
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly