Description:

Description:Celldeathorcytotoxicityisclassicallyevaluatedbythequantificationofplasmamembranedamage.Adenylatekinase(AK)isaubiquitousproteinpresentinalleukaryoticandprokaryoticcellsandrapidlyreleaseintoculturemediumupondamageoftheplasmamembrane.TheBioluminescenceCytotoxicityAssayKitisbasedonthemeasurementofAKinasimpleone-stepprocedureinvolvingtwochemicalreactions.ThefirstreactionistheconversionofADPtoATPbyadenylatekinasethatwasreleasedfromthedamagedcells.ThesecondreactionutilizesluciferasetocatalyzetheformationoflightfromATPandluciferin,andthelightcanbemeasuredusingaluminometerorbetacounter.Theassayishighlysensitiveandcanbefullyautomaticforhighthroughput.
Summary:
-Detectionmethod-Luminescence
-Sampletype-Cellculture(adherentandsUSPension)
-Speciesreactivity-Mammalian
Kitcomponents:
-AKDetectionReagent(Lyophilized)
-AKAssayBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takeslessthan30minutes
-Fastandconvenient
-Theassayishighlysensitiveandcanbefullyautomaticforhighthroughputstudies.
ShippingConditions:Gelpack

AdditionalInformation:

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Name	BioluminescenceCytotoxicityAssayKit
RelatedProductNames	BioluminescenceCytotoxicityAssayKit,Cytotoxicityassay,CytotoxicityAssayKit,Adenylatekinase,AKassay
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly