Description:

Description:ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.TheCaspase-10FluorometricAssayKitprovidesasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceAEVD.TheassayisbasedondetectionofcleavageofsubstrateAEVD-AFC(AFC:7-amino-4-trifluoromethylcoumarin).AEVD-AFCemitsbluelight(λmax=400nm);uponcleavageofthesubstratebycaspase-10orrelatedcaspases,freeAFCemitsayellow-greenfluorescence(λmax=505nm),whichcanbequantifiedusingafluorometerorafluorecencemicrotiterplatereader.
Summary:
-Detectionmethod-Fluorescence(Ex/Em400nm/505nm)andfluorescencemicroscopy
-Sampletype-Cellandtissuelysates
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100,200,400assays)
-Applications-Detectearly/middlestagesofapoptosis;differentiateapoptosisfromnecrosis.
Kitcomponents:
-CellLysisBuffer
-2XReactionBuffer
-AEVD-AFC(1mM)
-DTT(1M)
FeaturesandBenefits:
-Simpleone-stepprocedure;takesonly1-2hours
-Fastandconvenient
-ComparisonofthefluorescenceofAFCfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseinAEVD-dependentcaspaseactivity.
ShippingConditions:Gelpack

AdditionalInformation:

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Name	Caspase-10FluorometricAssayKit-200assays
RelatedProductNames	Caspase-10FluorometricAssayKit,Caspase-10,caspase10,caspase10,Caspase-10Substrate,AVED,AEVD-AFC,Caspase-10assay,Caspase-10FluorometricAssay
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly