Description:

Description:ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.TheCaspase-2ColorimetricAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceVDVAD.Theassayisbasedonspectrophotometricdetectionofthechromophorep-nitroanilide(pNA)aftercleavagefromthelabeledsubstrateVDVAD-pNA.ThepNAlightemissioncanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat400-or405nm.
Summary:
-Detectionmethod-Absorbance(400or405nm)
-Sampletype-Cellandtissuelysates
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100,200,400assays)
-Applications-Detectearly/middlestagesofapoptosis;differentiateapoptosisfromnecrosis.
Kitcomponents:
-CellLysisBuffer
-2XReactionBuffer
-VDVAD-pNA(4mM)
-DTT(1M)
-DilutionBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takes1-2hours
-Fastandconvenient
-ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-2activity.
ShippingConditions:Gelpack

AdditionalInformation:

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Name	Caspase-2ColorimetricAssayKit-100assays
RelatedProductNames	Caspase-2ColorimetricAssayKit,Caspase-2,caspase2,caspase2,Caspase-2Substrate,VDVAD,VDVAD-pNA,Caspase-2assay,Caspase-2ColorimetricAssay
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly