Description:

Short Description: Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1

Additional Information:

Name	Human ICAM-1 ELISA Kit
Related Product Names	BB2|CD 54|CD54|CD54 antigen|Cell surface glycoprotein P3.58|Human rhinovirus receptor|ICAM 1|Intercellular adhesion molecule 1|Major group rhinovirus receptor|MALA2|MGC6195|MyD10|P3.58|Surface antigen of activated B cells
Gene Symbol	ICAM1
Gene Fullname	intercellular adhesion molecule 1
Gene id	3383
Alias Symbols	Intercellular adhesion molecule 1, Major group rhinovirus receptor, CD54
Protein Name	Intercellular adhesion molecule 1
Description of Target	Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellula
Swissprot ID	P05362
Protein Accession Num	NP_000192.2
Nucleotide Accession Num	NM_000201.2
Species Reactivity	Human
Sensitivity	< 10="">
Kit Range	156 pg/ml-10,000 pg/ml
Immunogen	NSO, Q28-E480
Homology	No detectable cross-reactivity with any other cytokine.
Standard Range	156pg/ml-10,000pg/ml. Test has a sensitivity of < 10="">
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA)
Specimen/Volumes	Refer to Manual
Swiss Prot Number	P05362
Specificity	There is no detectable cross-reactivity with other relevant proteins.
Concentration	Lot Specific
Applications	ELISA
GI Number	3383
Stability	Four months at 4C and eight months at -20C.
Reconstitution and Storage	Store at 4C for frequent use, at -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity	Reactive to Human. There is no detectable cross-reactivity with other relevant proteins.
Kit Size	1x96 well plate
Kit Principle	Genway Biotech ICAM1 ELISA Kit (Human) (GWB-ZZD043) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for ICAM1 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, Q28-E480) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human ICAM1 captured in well.
Kit Component	Component Amount Lyophilized recombinant mouse Fractalkine standard 10 ng/tube x 2 Anti-mouse Fractalkine Antibody Well Plate 96 Wells Sample diluent buffer 30 mL Biotinylated anti- mouse Fractalkine antibody 130 uL, dilution 1:100 Antibody diluent buffer 12 mL Avidin-Biotin-Peroxidase Complex (ABC) 130 uL, dilution 1:100 ABC diluent buffer 12 mL TMB color developing agent 10 mL TMB stop solution 10 mL
Kit Detection	Colorimetric, OD 450 nm
Kit Duration	~ 3 Hours
Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Lead Time	Domestic: within 1-2 weeks delivery International: 1-2 weeks
Intended Use	Research Use Only
Key Reference	1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. Assignment of the gene for intercellular adhesion molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991. 2. Bella, J.; Kolatkar, P. R.; Marlor, C. W.; Greve, J. M.; Rossmann, M. G. The structure of the two amino-terminal domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc. Nat. Acad. Sci. 95: 4140-4145, 1998. 3. Simmons, D.; Makgoba, M. W.; Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM. Nature 331: 624-627, 1988.
Additional Information	Range: 156pg/ml-10,000pg/ml
::	Principle: Aviva’s human ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards (NSO,Q28-E480) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-1 amount of sample captured in plate.
::	Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended. 2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case. 3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes. 4. Duplicate well assay is recommended for both standard and sample testing. 5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate. 6. Don’t reuse tips and tubes to avoid cross contamination. 7. To avoid to use the reagents from different batches together. 8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using. Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, consisting of 455 amino acids with the molecular mass of 50kDa.

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