Description:

Short Description: In this assay the IgG2A present in serum sample reacts with the anti-IgG2A antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-IgG2A antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum IgG2A. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG2A in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG2A in the test sample. The quantity of IgG2A in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information:

Name	Mouse IgG2a ELISA Kit
Related Product Names	Mouse IgG2a ELISA Kit; Immunoglobulin heavy chain gamma polypeptide Mouse IgG2aIgG2a
Protein Name	IgG2A
Species Reactivity	Mouse
Assay Info	Assay Methodology: Quantitative Sandwich ELISA
Sensitivity	0.861 ng/ml
Kit Range	12.5 ng/ml - 400 ng/ml
Homology	Mouse
Standard Range	Refer To Manual
Sample Type	Serum, plasma and other biological fluids
Product Format	In Solution
Specimen/Volumes	Refer To Manual
Swiss Prot Number	P01863
Applications	ELISA
Reconstitution and Storage	1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-IgG2A conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-MOUSE IgG2A ELISA MICRO PLATE Anti-Mouse IgG2A coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. MOUSE IgG2A CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity	Mouse
Kit Size	96 well
Kit Principle	Genway Biotech IgG2A ELISA Kit (Mouse) (GWB-CF2E7B) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgG2A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgG2A - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG2A captured in the well.
Kit Component	Component Amount Anti-Mouse IgG2A Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Mouse IgG2A Calibrator 1
Kit Duration	90 Minutes
Storage	Refer to kit instructions for component storage
Lead Time	Domestic: within 1 week delivery International: 1 week
Intended Use	Research Use Only
Additional Information	Intended Use: The IgG2A test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG2A in biological samples of mice.
::	Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Mouse IgG2A antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-MOUSE IgG2A ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse IgG2A. 7. MOUSE IgG2A CALIBRATOR One vial containing Mouse IgG2A calibrator.

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