Description:

ProductName:Interleukin-8

Category:ImmunologyMarkers

SampleType:Serum

Sensitivity:1,1pg/mL

Description:ImmunoenzymetricassayfortheinvitroquantitativemeasurementofhumanInterleukin-8(IL-8)inplasma.

#ofSamples:1X96Assays

IntendedUse:Immunoenzymetricassayfortheinvitroquantitativemeasurementofhumaninterleukin-8(IL-8)inplasma.

Background:A.BIOLOGicalactivities
IL-8(alsoknownasNAP-1forNeutrophil-activatingpeptide)isachemoattractantproteinforneutrophils.Thiscytokinebelongstoanewfamilyofchemotacticpeptidescalled"chemokines".Thisproin¬ammatorymediatorissecretedbydierentcellssuchasmonocytes,neutrophils,endothelialcells,broblastafteractivation,andbymitogen-stimulatedTlymphocytes.TheroleofIL-8intherecruitmentofneutrophilsinthelungduringARDShasalsobeensuggested.

Principlesofthemethod:TheGenWayIL-8-EASIAisasolidphaseEnzymeAmplifiedSensitivityImmunoassayperformedonmicrotiterplate.Theassayusesmonoclonalantibodies(MAbs)directedagainstdistinctepitopesofIL-8.Calibratorsandsamplesreactwiththecapturemonoclonalantibody(MAb1)coatedonmicrotiterwellandwithamonoclonalantibody(MAb2)labelledwithhorserADIshperoxidase(HRP).Afteranincubationperiodallowingtheformationofasandwich:coatedMAb1-humanIL-8-MAb2-HRP,themicrotiterplateiswashedtoremoveunboundenzymelabelledantibody.Boundenzymelabelledantibodyismeasuredthroughachromogenicreaction.Chromogenicsolution(TMB)isaddedandincubated.ThereactionisstoppedwiththeadditionofStopSolutionandthemicrotiterplateisthenreadattheappropriatewavelength.Theamountofsubstrateturnoverisdeterminedcolourimetricallybymeasuringtheabsorbance,whichisproportionaltotheIL-8concentration.AcalibrationcurveisplottedandIL-8concentrationinsamplesisdeterminedbyinterpolationfromthecalibrationcurve.TheuseoftheEASIAreader(linearityupto3ODunits)andasophisticateddatareductionmethod(polychromaticdatareduction)resultinahighsensitivityinthelowrangeandinanextendedcalibrationrange.

StorageandStABIlity:•Beforeopeningorreconstitution,allkitscomponentsarestableuntiltheexpirydate,indicatedontheviallabel,ifkeptat2to8C.
•Unusedstripsmustbestored,at2-8C,inasealedbagcontainingadesiccantuntilexpirationdate.
•Afterreconstitution,calibrators,controlsandSpecimenDiluentarestablefor4daysat2to8C.Forlongerstorageperiods,aliquotsshouldbemadeandkeptat-20Cformaximum2months.Avoidsuccessivefreezethawcycles.
•TheconcentratedWashSolutionisstableatroomtemperatureuntilexpirationdate.
•FreshlypreparedWorkingWashsolutionshouldbeusedonthesameday.
•Afteritsfirstuse,theconjugateisstableuntilexpirydate,ifkeptintheoriginalwell-closedvialat2to8C.
•Thefreshlypreparedrevelationsolutionisstable,beforeuse,formaximum15minutesatroomtemperatureandmustbediscardedafterwards.
•Alterationsinphysicalappearanceofkitreagentsmayindicateinstabilityordeterioration.

SensitivityandSpecificity:Nosignificantcross-reactionwasobservedinpresenceof50ngofIL-1?,IL-1?,IL-1ra,IL-2,IL-3,IL-4,IL-6,IL-7,IL-10,TNF-?,TNF-?,IFN-?,IFN-?,IFN-?,TGF-?,GM-CSF,OSM,MIP-1?,MIP-1?,LIF,MCP-1,G-CSF,RANTES,PF-4,?TG,GRO,IP-10andSCF.ThisIL-8assayisspecificforhumannaturalandrecombinantIL-8andisabletorecognizethe72a.a.formofIL-8.

ProductName:Interleukin-8

Function:IL-8isachemotacticfactorthatattractsneutrophils,basophils,andT-cells,butnotmonocytes.Itisalsoinvolvedinneutrophilactivation.Itisreleasedfromseveralcelltypesinresponsetoaninflammatorystimulus.IL-8(6-77)hasa5-10-foldhigheractivityonneutrophilactivation,IL-8(5-77)hasincreasedactivityonneutrophilactivationandIL-8(7-77)hasahigheraffinitytoreceptorsCXCR1andCXCR2ascomparedtoIL-8(1-77),respectively.

Subunit:Homodimer.

SubcellularLocation:Secreted.

Ptm:SeveralN-terminalprocessedformsareproducedbyproteolyticcleavageaftersecretionfromatleastperipheralbloodmonocytes,leukcocytesandendothelialcells.Ingeneral,IL-8(1-77)isreferredtoasinterleukin-8.IL-8(6-77)isthemostpromimentform.

Similarity:Belongstotheintercrinealpha(chemokineCxC)family.

AdditionalInformation:

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Name	Interleukin-8ELISAKit
RelatedProductNames	Interleukin-8ELISAKit;IL-8;C-X-Cmotifchemokine8;Monocyte-derivedneutrophilchemotacticfactor;MDNCF;T-cellchemotacticfactor;Neutrophil-activatingprotein1;NAP-1;Protein3-10C;Granulocytechemotacticprotein1;GCP-1;Monocyte-derivedne
NCBIAccNumber	NP_000575.1
MolecularWeight	11098
SwissProtNumber	P10145
IntendedUse	ResearchUseOnly