Description:

PleaseclickhereforMSDSPDFDatasheet

ProductName:ChlamydiapneumoniaeIgM–ELISA

#ofSamples:1x96Assays

IntendedUse:TheChlamydiapneumoniaeIgM-ELISAisintendedforthequalitativedeterminationofIgMclassantibodiesagainstChlamydiapneumoniaeinhumanserumorplasma(citrate).

Introduction:Chlamydiaearenomotile,Gramnegativeandobligatoryintracellulargrowingbacteriawhichformcharacteristicinclusionswithinthecytoplasmofparasitizedcells.TheyareeasilyvisIBLeinthelightmicroscope.ThreedifferentChlamydiaspeciespathogenicforhumansareknown:Chlamydiatrachomatis,ChlamydiapneumoniaeandChlamydiapsittaci,andonespeciesonlypathogenicforanimals(C.pecorum).Chlamydiatrachomatisisthemostprevalentagentofsexuallytransmitteddiseasesworldwide(400-500millioncases)andthenumberofinfectionsisconstantlygrowing.PregnantwomeninfectedwithC.trachomatismaytransmitthesebacteriaduringchildbirth,causingconjunctivitisorpneumoniainnewborns.Untreatedcasesofchlamydialinfectioncanleadtochronicsalpingitis,possiblyresultinginectopicpregnancyorinfertility.Inmales,C.trachomatisisamajorcauseofnon-gonococcalurethritis.AsevereprobleminChlamydiainfectionsisthefrequentasymptomaticinsidiouscoursewhichmayresultintheinitiationofchronicdiseases.Inmanyinstancesprimaryinfectionsarenotrecognizedandonlythesequelaecausedbyascended,persistingagentsarediagnosed.
Infectionmaybeidentifiedby
Microscopy:Giemsastain
PCR
SEROlogy:DetectionofantigensbyELISA
DetectionofantibodiesbyIF,EIA,ELISA

Principlesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgM-classantibodiesagainstChlamydiapneumoniaeisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.
MicrotiterstripwellsareprecoatedwithChlamydiapneumoniaeantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgMconjugateisadded.ThisconjugatebindstothecapturedChlamydiapneumoniae-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofChlamydiapneumoniae-specificIgMantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.

StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.

LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunsuppremizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.
TheChlamydiapneumoniaeantigenwhichiscoatedontheplatesiscomprisedofelementarybodies.AcrossreactionwithChlamydiatrachomatiscannotbeexcludedwithseracontainingantibodiestoLPSandMOMP.

ReferencesHoymeU.B.,SpitzbartH.(1996).PastandcurrentprevalenceofChlamydiatrachomatisinwomeninGermany.In:ChlamydiaResearch.AngelikaStary(ed.).ProceedingsofthethirdmeetingoftheEuropeanSocietyforChlamydiaResearch,Vienna,Austria,11.-14.Sept.p.391.PaavonenJ.(1996).Chlamydiatrachomatis:Amajorcauseofmucopurulentcervicitisandpelvicinflammatorydiseaseinwomen.In:SexuallyTransmittedDiseases.AdvancesinDiagnosisamdTreatment.Curr.Probl.Dermatol.ElsnerP.,EichmannA.(eds.),Basel,Karger,Vol.24,pp.110-122.PetersenE.E.,CladA.(1995).GenitaleChlamydieninfektionen.DeutschesÄrzteblatt92,Heft5,A-277-282.WeströmL.(1996).ConsequencesofgenitalChlamydiainfectionsinwomen.In:ChlamydiaResearch.AngelikaStary(ed.).ProceedingsofthethirdmeetingoftheEuropeanSocietyforChlamydiaResearch,Vienna,Austria,11.-14.Sept.pp.137-140.WeströmL.V.(1996).Chlamydiaanditseffectonreproduction.J.Brit.Fertil.Soc.1:23-30.

AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

Name	ChlamydiapneumoniaeIgM
RelatedProductNames	ChlamydiapneumoniaeIgM–ELISA
MolecularWeight	0.5
Storage	Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.
IntendedUse	ResearchUseOnly