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Name	RabbitPlasminogenELISAKit
RelatedProductNames	RabbitPlasminogenELISAKit
ProteinName	Plasminogen
SpeciesReactivity	Rabbit
AssayInfo	AssayMethodology:QuantitativeSandwichELISA
Sensitivity	0.905ng/ml
KitRange	6.25ng/ml-200ng/ml
Homology	Rabbit
StandardRange	ReferToManual
SampleType	Serum,plasmaandotherBIOLOGicalfluids
ProductFormat	InSolution
Specimen/Volumes	ReferToManual
Applications	ELISA
ReconstitutionandStorage	1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE UndilutedhorserADIshperoxidaseanti-PMGconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-RABBITPLASMINOGENELISAMICROPLATE Anti-RabbitPMGcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.RABBITPLASMINOGENCALIBRATOR LongTermStorage:Uponreceipt,aliquotthecalibratorandstorethemfrozen.Theywillbestableuntilexpirationdate.ShortTermStorage:thecalibratorisstableforupto14daysat4C.Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
Reactivity	Rabbit
KitSize	96well
KitPrinciple	GenWayBiotechPlasminogenELISAKit(Rabbit)(GWB-344550)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforPlasminogenhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Plasminogen-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsamplePlasminogencapturedinthewell.
KitComponent	Component Amount Anti-RabbitPlasminogenMicroplate 96Wells(12x8WellStrips) 20XDiluentConcentrate 50mL 20XWashSolution 50mL 100XEnzyme-AntibodyConjugate 150uL Chromogen-SubstrateSolution 12mL StopSolution 12mL RabbitPlasminogenCalibrator 1
KitDuration	90Minutes
Storage	Refertokitinstructionsforcomponentstorage
LeadTime	Domestic:within1weekdelivery International:1week
IntendedUse	ResearchUseOnly
AdditionalInformation	IntendedUse:ThePlasminogentestkitisahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringPlasminogeninbiologicalsamplesofrabbit.
::	Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-RabbitPlasminogenantibodyconjugated withhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-RABBITPLASMINOGENELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RabbitPMG. 7.RABBITPLASMINOGENCALIBRATOR OnevialcontainingRabbitPlasminogencalibrator.