Description:

ShortDescription:Inthisassaythealbuminpresentinserumsamplereactswiththeanti-Alb.antibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-Alb.antibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumAlb.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofAlbumininthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofAlbumininthetestsample.ThequantityofAlbumininthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.

AdditionalInformation:

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Name	CanineAlbuminELISAKit
RelatedProductNames	DogAlbuminELISAKit;AllergenCanf3DogAlbuminALB
Geneid	403550
AliasSymbols	Alphafetoglobulin
ProteinName	Serumalbumin
DescriptionofTarget	Serumalbumin,themainproteinofplasma,hasagoodbindingcapacityforwater,Ca2+,Na+,K+,fattyacids,hormones,bilirubinanddrugs.Itsmainfunctionistheregulationofthecolloidalosmoticpressureofblood.Majorzinctransporterinplasma,
SwissprotID	P49822
SpeciesReactivity	Dog
AssayInfo	AssayMethodology:QuantitativeSandwichELISA
Sensitivity	2.463ng/ml
KitRange	12.5ng/ml-400ng/ml
Homology	Dog
StandardRange	ReferToManual
SampleType	Serum,plasmaandotherBIOLOGicalfluids
ProductFormat	InSolution
Specimen/Volumes	ReferToManual
SwissProtNumber	P49822
Applications	ELISA
ReconstitutionandStorage	1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE Undilutedhorseradishperoxidaseanti-Albuminconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-DOGALBUMINELISAMICROPLATE Anti-DogAlbumincoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.DOGALBUMINCALIBRATOR ThelyophilizedDogAlbumincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
Reactivity	Canine
KitSize	96well
KitPrinciple	GenWayBiotechAlbuminELISAKit(Dog)(GWB-1BB207)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforSerumalbuminhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Serumalbumin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleSerumalbumincapturedinthewell.
KitComponent	Component Amount Anti-DogAlbuminMicroplate 96Wells(12x8WellStrips) 20XDiluentConcentrate 50mL 20XWashSolution 50mL 100XEnzyme-AntibodyConjugate 150uL Chromogen-SubstrateSolution 12mL StopSolution 12mL DogAlbuminCalibrator 1
KitDuration	100Minutes
Storage	Refertokitinstructionsforcomponentstorage
LeadTime	Domestic:within1weekdelivery International:1week
IntendedUse	ResearchUseOnly
AdditionalInformation	IntendedUse:TheAlbumintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringAlbumininbiologicalfluidofDogs.
::	Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-DogAlbuminantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-DOGALBUMINELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-DogAlbumin. 7.DOGALBUMINCALIBRATOR OnevialcontainingalyophilizedDogAlbumincalibrator.