FluorescentDOPC/CHOLliposomeslabeledwithFluoresceinDHPE(200nm)
HighqualityLUVliposomeslabeledwithfluoresceinDHPE are readytouse.Theliposomesarepreparedbytheextrusiontechnologyto200-250nmnmwithtightparticlesizedistribution. Theproductissemisterilefilteredthrough0.45umfiltersandfilledinautoclavedvialsguaranteedtobestablefor3monthsstoredinrefrigerator. Thephospholipid,fluoresceinDHPEislabeledontheheadgroupwiththegreen-fluorescentpH-sensitivefluorophore,fluorescein(excitation/emissionmaxima~496/519nm).
Specifications
Productcode:F60103F2-F
Lipidcomposition:DOPC/CHOL/Fluorescein-DHPE(54:45:1mol/mol)
Lipidconcentration:50mM(50-55mM)
FluorsceinDHPE: 0.5mM(0.59mg/mL)
Meanparticlesize: 230nm(200-250nm,intensityweightedlognormalperDSL)
Particlesizedistribution: 80-100nm(half-width)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.5±0.2
Storage: in refrigerator(2-8degreeC)
Shelf-life: 3monthforunopenedvials,protectfromlight
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL:cholesterol
FluoresceinDHPE:N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine,triethylammoniumsalt (InvitrogenF-362)