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FabGennix/StainODye Nitrocellulose Membrane Protein Stain/FGI-1933

价格
¥3000.00
货号:FGI-1933
浏览量:127
品牌:FabGennix
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商品描述
  • Description

Overview

Product

StainODyeTM

Description

The FabGennix StainODyeTM is a Reversible Protein Stain for Nitrocellulose Membranes is an efficient and accurate alternative to Ponceau S stain for quantiating the transfer efficiency of proteins on nitrocellulose/charged membranes.

Volume

200 ML

Concentration

Ready-to-use solution

Storage

Room temperature

Applications

25 mL of SainODyeTM can be used on 25-50 mini-blots.

Advantages

  • Quickly determine the transfer efficiency of proteins on pure nitrocellulose/supported nitrocellulose and charged membranes
  • Avoid re-doing western blots due to uneven transfer, non-transfer and air bubbles
  • Stained membranes can be photographed for quality control step in the Western blotting protocol.
  • Compatible with Nitrocellulose and PVDF membranes
  • Preservatives allow for a shelf-life of 1 year at room temperature

StainODyeTM is a reversible and sensitive tool to detect transfer efficiencies of protein on nitrocellulose membranes. With fast response time, lower limit of detection of 25-50 ng per band and a quick and easy protocol StainODyeTM is a better alternative to traditional Ponceau S staining. Unlike other stains like Amido black/Coomassie, which permanently binds to proteins, StainODyeTM has a high affinity for protein, but is washed off in PBS or blocking solutions. Additionally StainODyeTM labels the glycosylated proteins with high avidity resulting visualization of total proteins.

Quantitative Western blotting applications requires uniform transfer of proteins to membrane for comparison analyses of results. There are accurate methods to load a fixed quantity of proteins on the gel before electrophoresis, however, few methods are available to quantitate the transfer efficiency of proteins on the nitrocellulose/charged membranes. Uneven transfer, non-transfer and air bubbles are some of the problem that can compromise quantitative western-blotting results. The StainODyeTM allows direct visualization of proteins on nitrocellulose or other types of transfer media immediately after transfer of proteins to charged membranes. The protein bands appear red or pink after membrane is developed in StainODyeTM. These stained membranes can be photographed for permanent records. The StainODyeTM is reversible and is washed off by rinsing the blots in water or PBS. Alternatively, they can be directly incubated in blocking buffer (BlockOBufferTM) for western blotting. The StainODyeTM comes with preservatives for an approximate shelf life of 1 year at room temperature.

Protocol

  1. Pour approximately 10-15 mL dye for 10 x 12 cm membranes in a clean glass container.
  2. Incubate the membrane by slow shaking in a rocking platform for 1-2 minutes.
  3. Remove and discard the excess dye and wash membranes with distilled water or PBS until the background is clear.
    1. Several washes may be necessary for optimal signal to noise ratios.
  4. The membrane can be photographed immediately, dry membranes can be photographed by wetting in water.
  5. There may be slight fainting of bands upon long-term storage. Best results are obtained by immediately recording the color intensity

Fabgennix 品牌介绍 官网:http://www.fabgennix.com/   Fabgennix公司是一家知名的抗体研发生产企业,为全球生物技术公司、科研单位和生物制药公司提供一抗、二抗、试剂、试剂盒、多肽和阳性质控品等产品。 基于坚实的科研背景,Fabgennix公司已成为全球免疫诊断市场高质量单克隆抗体的优质供应商。       Fabgennix 主要产品分类     【一抗】 1. 癌症研究 2. 免疫学 3. 微生物学 4. 信号转导 5. 新陈代谢和内稳态 6. 视力和嗅觉 7. 通道和转运蛋白 8. 表观遗传学 9. 干细胞 10. 细胞生物学 11. 神经病学 12. 心脏病学 13. 内分泌系统 【二抗】 1. Rabbit Anti-Chicken IgG HRP 【试剂】 1. Western Blot Stripping Buffer 2. 硝酸纤维膜蛋白染料 【试剂盒】 1. 细胞溶解试剂盒 2. PDEase 试剂盒 3. 外周血单核细胞(PBMC)分离试剂盒 【实验室服务】 1. 客户抗体显影 2. 蛋白化学多肽合成实验室 3. 免疫学实验室 4. 影像处理实验室 5. 免疫组化实验室